Sensitive method for the determination of bisphenol-A in serum using two systems of high-performance liquid chromatography
Sensitive method for the determination of bisphenol-A in serum using two systems of high-performance liquid chromatography
复制标题
DOI：
10.1016/s0378-4347(99)00471-5
复制
发表时间：
1999-12-24
影响因子：
3
通讯作者：
Yonekubo, J
中科院分区：
文献类型：
--
作者：
Sajiki, J;Takahashi, K;Yonekubo, J
本研究的目的是建立一种简便、准确的测定血清和尿液等体液中双酚a （BPA）含量的方法。采用高效液相色谱(HPLC) -电化学检测器（ED）和高效液相色谱-电喷雾质谱（MS）两种高效液相色谱（HPLC）系统对固相萃取法制备的血清样品进行分析。在50%以下的水或乙醇浓度下提取血清中的BPA是合适的。HPLC-ED法和HPLC-MS法的双酚a检出限分别为0.2 ng ml（-1）和0.1 ng ml（-1）。两种高效液相色谱系统的测定结果具有良好的相关性。健康人血清中BPA浓度较低（0-1.6 ng ml(-1)）。然而，从各种商业胎牛血清和羊血浆中检测到大量的双酚a。由于采集后羊血浆中未立即检测到双酚a，因此高含量的双酚a被认为是在采集后制备产品过程中处理血液造成的。体外研究表明，从聚碳酸酯管中浸出的BPA进入绵羊血浆的量是进入水中的BPA的40倍，并且浸出量与温度（37℃>20℃>5℃）有关。(C) 1999 Elsevier Science B.V.。
The aim of this study was to establish an easy and accurate method for the determination of bisphenol-A (BPA) in the body Liquid such as serum and urine. Two high-performance liquid chromatography (HPLC) systems, HPLC with electrochemical detector (ED), and HPLC with mass spectrometry (MS) using electrospray ionization (ESI) interface were used for the assay in the serum samples prepared with solid-phase extraction method. Water or EtOH at a concentration below 50% was suitable for the extraction of BPA from serum. The limit of detection of BPA was 0.2 ng ml(-1) for the HPLC-ED method and 0.1 ng ml(-1) for HPLC-MS. There was a good correlation between the data obtained by the two HPLC systems. BPA concentrations in healthy human serum were low (0-1.6 ng ml(-1)). From various commercial fetal bovine serum and sheep plasma, however, significant amounts of BPA were detected. Since no BPA was detected from sheep plasma immediately after collection, the high amounts of BPA were considered to be caused by the handling of blood during the preparation of the products after blood collection. in vitro study showed that the amount of BPA leached from polycarbonate tube into sheep plasma were 40 times larger than those into water and the leached amount of BPA depended on the temperature (37 degrees C>20 degrees C>5 degrees C). (C) 1999 Elsevier Science B.V. All rights reserved.