EVALUATION OF 6 ANTIBODIES FOR IMMUNOHISTOCHEMISTRY OF MUTANT P53 GENE-PRODUCT IN ARCHIVAL COLORECTAL NEOPLASMS
EVALUATION OF 6 ANTIBODIES FOR IMMUNOHISTOCHEMISTRY OF MUTANT P53 GENE-PRODUCT IN ARCHIVAL COLORECTAL NEOPLASMS
复制标题
DOI：
10.1002/path.1711720104
复制
发表时间：
1994-01-01
影响因子：
7.3
通讯作者：
HAMILTON, SR
中科院分区：
文献类型：
--
作者：
BAAS, IO;MULDER, JWR;HAMILTON, SR
免疫组织化学检测核内P53基因产物可能提示染色体17p上的P53抑制基因突变。我们用6种市售的P53抗体对19例大肠肿瘤进行免疫组织化学染色，并与P53基因突变状态和17p等位基因缺失状态进行比较。根据Friedman的等级分析，使用小鼠单抗DO7与Target Unmamming iquid(TUF)进行抗原修复是最敏感和特异的操作(使用CAS 200图像分析仪P30%)有P53突变。无表达的七种肿瘤(李
Immunohistochemical detection of intranuclear p53 gene product may indicate mutation of the p53 suppressor gene on chromosome 17p. We used six commercially available antibodies for p53 immunohistochemistry on 19 archival colorectal neoplasms and compared the results with the mutation status of the p53 gene and 17p allelic deletion status. By Friedman's ranking analysis, use of mouse monoclonal antibody DO7 with Target Unmasking Fluid (TUF) for antigen retrieval was the most sensitive and specific procedure (P30 per cent using a CAS 200 image analyser) had p53 mutation. Of seven tumours without expression (LI