Differences in milk fat globule membrane proteins among Murrah, Nili-Ravi and Mediterranean buffaloes revealed by a TMT proteomic approach.
Differences in milk fat globule membrane proteins among Murrah, Nili-Ravi and Mediterranean buffaloes revealed by a TMT proteomic approach.
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DOI：
10.1016/j.foodres.2020.109847
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发表时间：
2021-01
影响因子：
8.1
通讯作者：
Shanshan Li;Yongxin Yang;Chen Chen-Chen;Ling Li;T. Valencak;D. Ren
中科院分区：
文献类型：
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作者：
Shanshan Li;Yongxin Yang;Chen Chen-Chen;Ling Li;T. Valencak;D. Ren
乳脂球膜蛋白（MFGM）对人体营养和健康有重要作用。然而，水牛品种间MFGM蛋白组的差异尚不清楚。本研究采用串联质量标签蛋白质组学方法，对Murrah、Nili-Ravi和地中海水牛的MFGM蛋白进行了鉴定，以表征其成分及其潜在活性。共鉴定出1258个MFGM蛋白，并在水牛品种中发现103个差异表达蛋白。其中，toll样受体2在地中海牛奶中含量更高；Nili-Ravi乳中内质网驻留蛋白29含量较高；尿调节素在Murrah牛奶中含量较高。水牛奶中的781种新蛋白质具有抗氧化、抗菌和抗炎活性。我们的研究结果揭示了不同水牛品种MFGM蛋白的组成复杂性和功能多样性。因此，我们的数据可能为促进生水牛奶作为一种健康和功能的乳制品铺平道路。
Milk fat globule membrane (MFGM) proteins contribute to human nutrition and health. However, differences in MFGM proteome among the buffalo breeds are unknown. In this study, MFGM proteins from Murrah, Nili-Ravi and Mediterranean buffaloes were identified using a tandem mass tag proteomic approach to characterize the components and their potential activities. A total of 1258 MFGM proteins were identified and 103 differentially expressed proteins were found in the studied buffalo breeds. Of these, toll-like receptor 2 had higher abundance in Mediterranean milk; endoplasmic reticulum resident protein 29 had higher levels in Nili-Ravi milk; uromodulin had higher abundance in Murrah milk. A number of 781 newly proteins in buffalo milk exerted antioxidant, antibacterial, and anti-inflammatory activities. Our findings reveal the component complexity and functional diversity of MFGM proteins in different buffalo breeds. Thus, our data may path the way to promote raw buffalo milk as a healthy and functional dairy product.