Molecular orientation of immunoglobulin G at high concentration on an ion-exchange sorbent.
Molecular orientation of immunoglobulin G at high concentration on an ion-exchange sorbent.
复制标题
高浓度免疫球蛋白 G 在离子交换吸附剂上的分子取向。
DOI：
10.1016/s0021-9673(00)94787-3
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Regnier,FE
中科院分区：
文献类型：
--
作者：
Mazsaroff,I;Cook,S;Regnier,FE
在整个蛋白质吸附等温线范围内，利用化学计量置换模型的推广，研究了结合在强阴离子交换表面上的免疫球蛋白分子取向的变化。Z数随固定相蛋白质浓度的增加而减小，接近一个极限值。建议用高度合作的完全相同结合部位内的多重平衡模型作为评价Z数随蛋白质浓度变化的一种可能方法。
The change of molecular orientation of IgG, bound on a strong-anion-exchange surface, was studied using a generalization of the stoichiometric displacement model, over the entire range of protein adsorption isotherms. The Z number was found to decrease with increasing stationary phase protein concentration, approaching a limiting value. The analogy of the multiple equilibria model within highly cooperative identical binding sites was suggested as a possible way to evaluate the observed change in Z number with the protein concentration.