Characterization of interaction of magnetic nanoparticles with breast cancer cells.
Characterization of interaction of magnetic nanoparticles with breast cancer cells.
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DOI：
10.1186/s12951-015-0073-9
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发表时间：
2015-02-26
影响因子：
10.2
通讯作者：
Carrascosa JL
中科院分区：
文献类型：
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作者：
Calero M;Chiappi M;Lazaro-Carrillo A;Rodríguez MJ;Chichón FJ;Crosbie-Staunton K;Prina-Mello A;Volkov Y;Villanueva A;Carrascosa JL
已经测试了不同的超顺磁性氧化铁纳米颗粒在癌症治疗中的潜在用途，因为它们以高效率进入细胞，不诱导细胞毒性，并且在细胞内保留相对长的时间。我们分析了二巯基琥珀酸包覆的超顺磁性氧化铁纳米粒子的相互作用，内化和生物相容性，平均直径为15 nm，负表面电荷在MCF-7乳腺癌细胞。将细胞与二巯基琥珀酸包被的超顺磁性氧化铁纳米颗粒孵育不同的时间间隔，范围为0.5至72小时。这些纳米颗粒表现出有效的内化和相对缓慢的清除。时间依赖性摄取的研究表明，最大的积累二巯基琥珀酸包被的超顺磁性氧化铁纳米粒子孵育24小时后，之后，他们慢慢地从细胞中除去。超顺磁性氧化铁纳米颗粒通过能量依赖性内吞作用内化并定位于内体中。透射电子显微镜研究表明，巨胞饮摄取和网格蛋白介导的内化取决于纳米颗粒聚集体的大小。MCF-7细胞积累这些纳米颗粒，而对细胞形态、细胞骨架组织、细胞周期分布、活性氧产生和细胞活力没有任何显著影响，显示出与未处理的对照细胞相似的行为。所有这些发现表明，二巯基琥珀酸包覆的超顺磁性氧化铁纳米粒子在应用于靶向乳腺癌细胞的效率和生物相容性方面具有优异的性能。本文的在线版本（doi：10.1186/s12951-015-0073-9）包含补充材料，可供授权用户使用。
Different superparamagnetic iron oxide nanoparticles have been tested for their potential use in cancer treatment, as they enter into cells with high effectiveness, do not induce cytotoxicity, and are retained for relatively long periods of time inside the cells. We have analyzed the interaction, internalization and biocompatibility of dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles with an average diameter of 15 nm and negative surface charge in MCF-7 breast cancer cells. Cells were incubated with dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles for different time intervals, ranging from 0.5 to 72 h. These nanoparticles showed efficient internalization and relatively slow clearance. Time-dependent uptake studies demonstrated the maximum accumulation of dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles after 24 h of incubation, and afterwards they were slowly removed from cells. Superparamagnetic iron oxide nanoparticles were internalized by energy dependent endocytosis and localized in endosomes. Transmission electron microscopy studies showed macropinocytosis uptake and clathrin-mediated internalization depending on the nanoparticles aggregate size. MCF-7 cells accumulated these nanoparticles without any significant effect on cell morphology, cytoskeleton organization, cell cycle distribution, reactive oxygen species generation and cell viability, showing a similar behavior to untreated control cells. All these findings indicate that dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles have excellent properties in terms of efficiency and biocompatibility for application to target breast cancer cells. The online version of this article (doi:10.1186/s12951-015-0073-9) contains supplementary material, which is available to authorized users.