Ultrasensitivedetermination of 5-methylcytosine and 5-hydroxymethylcytosine in genomic DNA bysheathless interfaced capillary electrophoresis-mass spectrometry.
Ultrasensitivedetermination of 5-methylcytosine and 5-hydroxymethylcytosine in genomic DNA bysheathless interfaced capillary electrophoresis-mass spectrometry.
复制标题
通过无鞘接口毛细管电泳-质谱法超灵敏测定基因组 DNA 中的 5-甲基胞嘧啶和 5-羟甲基胞嘧啶。
DOI：
10.1039/c5cc10155g
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发表时间：
2016
影响因子：
4.9
通讯作者：
Xin-Xiang Zhang
中科院分区：
文献类型：
--
作者：
Fang Yuan;Xiao-Hui Zhang;Ji Nie;Hong-Xu Chen;Ying-Lin Zhou;Xin-Xiang Zhang
利用多孔喷嘴，建立了一种新的毛细管电泳质谱无鞘界面，首次应用于胞嘧啶修饰的高灵敏检测。该系统仅用125pg(∼20细胞)基因组DNA样本就能很好地鉴定和定量5-甲基胞嘧啶和5-羟甲基胞嘧啶。
A newly developed sheathless interface for capillary electrophoresis–mass spectrometry, using a porous tip sprayer, was first applied for highly sensitive determination of cytosine modifications. The system performed well in identification and quantification of both 5-methylcytosine and 5-hydroxymethylcytosine using only 125 pg (∼20 cells) genomic DNA samples.