Compositional difference in middle ear effusion: Mucous versus serous
Compositional difference in middle ear effusion: Mucous versus serous
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DOI：
10.1097/00005537-200201000-00027
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发表时间：
2002-01-01
期刊：
影响因子：
2.6
通讯作者：
Yoon, JH
中科院分区：
文献类型：
--
作者：
Chung, MH;Choi, JY;Yoon, JH
目的：浆液性中耳炎通常对药物治疗有反应，而粘液性中耳炎对药物治疗不敏感。本研究旨在阐明浆液性和粘液性渗出的成分差异，并探讨粘液性中耳炎中耳粘膜中MUC5AC是否起主要粘蛋白的作用。研究设计本研究包括中耳积液的化学分析和中耳粘膜的免疫组织化学染色。方法：研究方法。收集27例粘液性中耳炎和18例浆液性中耳炎患者的中耳积液标本。用斑点印迹法或酶联免疫吸附试验检测粘蛋白、溶菌酶、分泌型免疫球蛋白A和白细胞介素8水平。对粘液性中耳炎中耳黏膜连续切片进行高碘酸席夫染色和抗MUC5AC单抗免疫组织化学染色。结果。粘液性积液比浆液性积液含有更高水平的粘蛋白、溶菌酶、分泌型免疫球蛋白A和白细胞介素8。免疫组织化学研究显示，粘液性中耳炎中耳粘膜仅有一小部分杯状细胞表达MUC5AC粘蛋白。结论：浆液性分泌物和粘蛋白均可增加中耳积液的粘滞性，MUC5AC以外的其他粘蛋白可能在中耳积液的粘度中起主要作用。有必要对渗出性中耳炎中耳积液中的主要粘蛋白进行进一步的研究。
Objectives: Serous otitis media is usually responsive to medical treatment, whereas mucoid otitis media is not. The present study was undertaken to elucidate the compositional difference between serous and mucoid effusion and to investigate whether MUC5AC acts as a major mucin in the middle ear mucosa with mucoid otitis media. Study Design This study involved a chemical analysis of middle ear effusion and immunostaining of the middle ear mucosa. Methods. Middle ear effusion samples were collected from 27 patients with mucoid otitis media and 18 patients with serous otitis media. The levels of mucin, lysozyme, secretory immunoglobulin A, and interleukin-8 were measured by dot blotting or enzyme-linked immunosorbent assay. Periodic acid-Schiff and immunohistochemical staining with monoclonal anti-MUC5AC antibody were performed on the serial sections of middle ear mucosa with mucoid otitis media. Results. Mucoid effusions contained higher levels of mucin, lysozyme, secretory immunoglobulin A, and interleukin-8 than did serous effusions. Immunohistological study revealed that MUC5AC mucin was expressed in only a small portion of the goblet cells of middle ear mucosa with mucoid otitis media. Conclusions: The study suggests that both serous secretions and mucin might make the middle ear effusion more viscous and that mucins other than MUC5AC might have a major role in the viscosity of middle ear effusion. Further study is necessary to identify the major mucins in the middle ear effusion of otitis media with effusion.