Application of QUIZ for GM quantification in food
Application of QUIZ for GM quantification in food
复制标题
QUIZ 在食品中转基因定量的应用
DOI：
10.1016/j.foodchem.2010.10.002
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发表时间：
2011
期刊：
影响因子：
8.8
通讯作者：
David Lee
中科院分区：
文献类型：
--
作者：
M. La Mura;Theodore R. Allnutt;A. Greenland;I. Mackay;David Lee
可以在不需要认证标准物质(CRM)的情况下使用QUZ(使用信息性零进行量化)来量化样品中的转基因。在这项工作中，我们应用测验来估计含有一种或两种转基因的加工食品中抗草甘膦除草剂™大豆和MON810的含量。结果表明，转基因材料的导出值与已知输入的值具有很好的一致性，并与实时定量聚合酶链式反应的结果相比较。
The quantification of GM in samples can be performed without the need for certified reference materials (CRMs) using QUIZ (quantitation using informative zeros). In this work we have applied QUIZ to estimate the contents of RoundUp Ready™ soya and MON810 in processed food containing one or both GMs. Results show good agreement between derived values and known input of GM material and compare favourably with quantitative real-time PCR.