Infrared Spectroscopy-Based Metabolomic Analysis for the Detection of Preharvest Sprouting in Grain
Infrared Spectroscopy-Based Metabolomic Analysis for the Detection of Preharvest Sprouting in Grain
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DOI：
10.1094/cchem-01-16-0005-r
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发表时间：
2016-09-01
期刊：
影响因子：
2.4
通讯作者：
Jones, Oliver A. H.
中科院分区：
文献类型：
--
作者：
Burke, Matthew;Small, Darryl M.;Jones, Oliver A. H.
谷物收获前发芽给种植者和购买者造成重大损失。这个问题在世界各地都有发生，经济损失可能高达数百万美元。对发芽的目视评估是无效的，因为即使谷物没有显示出外部迹象（例如，具有可见的芽），也可能已充分发芽，因此不再可能用它们制成高质量的产品。目前确定谷物是否发芽的程序是基于测量酶或淀粉水平。然而，尽管过去 50 年来人们一直致力于开发增强的发芽测量方法，但目前还没有足够准确或可靠的方法。目前的工作评估了基于红外光谱的代谢组学分析评估大麦和小麦发芽的潜力。结果表明，中红外和近红外光谱能够确定谷物是否已经发芽，并指示发芽过程发生了多长时间。这两种技术已经在谷物行业中得到了很好的应用，并且有可能成为简单、快速的码头测试的基础，从而可以分离出完好的和轻度发芽的谷物。
Preharvest sprouting of grains causes significant losses to growers and buyers. The problem occurs throughout the world, and economic losses can be millions of dollars. Visual assessment of germination is ineffective because even grains showing no external signs (e.g., having a visible shoot) may still have germinated sufficiently so that it is no longer possible for high-quality products to be made from them. Current procedures to determine whether a grain has germinated are based around measuring enzyme or starch levels. However, despite protracted efforts to develop enhanced ways to measure germination over the last 50 years, there are currently no sufficiently accurate or reliable approaches available. The present work assesses the potential for infrared spectroscopy-based metabolomic profiling to assess the germination of barley and wheat. The results indicate that mid-and near-infrared spectroscopy are able to determine if the grain has germinated and give an indication of how long the germination process has been occurring. Both techniques are already well established in the grain industry and have the potential to form the basis of a simple, fast dockside test that would allow segregation of sound and mildly sprouted grain.