Differential gene and protein expression in gastrocnemius and tibialis anterior muscle following tibial and peroneal nerve injury in rats
Differential gene and protein expression in gastrocnemius and tibialis anterior muscle following tibial and peroneal nerve injury in rats
复制标题
大鼠胫腓神经损伤后腓肠肌和胫骨前肌差异基因和蛋白表达
DOI：
10.1016/j.gep.2019.119079
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发表时间：
2020-01-01
影响因子：
1.2
通讯作者：
Lin, Haodong
中科院分区：
文献类型：
--
作者：
Lin, Yaofa;Xie, Zheng;Lin, Haodong
周围神经损伤在临床上比较常见，治疗效果往往不理想。因此，促进神经再生和功能恢复是神经科学研究的首要目标。周围神经损伤后相应靶肌肉的恢复情况可能不同，但原因尚不清楚。在此，我们用RNA测序和蛋白质组学方法研究了胫骨和腓总神经损伤后腓肠肌和胫前肌的差异基因和蛋白表达，并用生物信息学方法对结果进行了分析。总共在术后第1、7、14和21天的腓肠肌和胫前肌中发现了1794、1765、1656和2006年的差异基因和398、400、959和472个差异蛋白，与51条信号通路的激活有关。这些基因和蛋白的差异表达可能有助于周围神经损伤后靶器官的恢复程度。这些发现为研究周围神经损伤后的再生机制提供了基础。
Peripheral nerve injury is encountered quite commonly in the clinic, and treatment results are often not satisfactory. Therefore, promoting nerve regeneration and functional recovery is a primary goal of neuroscience research. Recovery of corresponding target muscle can differ following peripheral nerve injury, but the reasons are unknown. Herein, we investigated differential gene and protein expression in gastrocnemius and tibialis anterior muscle following tibial and common peroneal nerve injury using RNA sequencing and proteomics approaches, and analysed the results by bioinformatics. In total, 1794, 1765, 1656 and 2006 differential genes and 398, 400, 959 and 472 differential proteins were identified in gastrocnemius and tibialis anterior muscles at 1, 7, 14 and 21 days after surgery, related to activation of 51 signalling pathways. Differential expression of these genes and proteins may contribute to the degree of recovery of target organs following peripheral nerve injury. The findings provide a foundation for investigating regeneration mechanisms following peripheral nerve injury.