Tributyltin inhibits osteoblastic activity and disrupts calcium metabolism through an increase in plasma calcium and calcitonin levels in teleosts.
Tributyltin inhibits osteoblastic activity and disrupts calcium metabolism through an increase in plasma calcium and calcitonin levels in teleosts.
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DOI：
10.1016/j.lfs.2005.10.004
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发表时间：
2006-04
期刊：
影响因子：
6.1
通讯作者：
N. Suzuki;M. Tabata;A. Kambegawa;A. Srivastav;A. Shimada;H. Takeda;Masaki Kobayashi;S. Wada;T. Katsumata;A. Hattori
中科院分区：
文献类型：
--
作者：
N. Suzuki;M. Tabata;A. Kambegawa;A. Srivastav;A. Shimada;H. Takeda;Masaki Kobayashi;S. Wada;T. Katsumata;A. Hattori
为了研究醋酸三丁基锡(TBTA)对破骨细胞和成骨细胞的直接作用，本研究使用了一种既具有破骨细胞又具有成骨细胞的硬骨鳞片，它类似于哺乳动物的膜骨。抗酒石酸酸性磷酸酶和碱性磷酸酶的活性分别作为两种细胞活性的指标。在淡水硬骨鱼和海洋硬骨鱼中，鳞片中的破骨活性没有因为四丁基三氮唑处理(10−9到10−5M)而改变。而在孵育6h后，金鱼、金枪鱼和海豚的成骨细胞活性均下降。在金鱼体内，即使10−~10M的四丁基三甲胺也显著抑制成骨细胞的活性。金鱼的抑制活性明显强于金鱼和海鱼。因此，我们用金鱼研究了这一机制的细节。参与成骨细胞生长和分化的雌激素受体和胰岛素样生长因子-I的mRNA表达在TBTA处理的鳞片中降低。然而，金属硫蛋白(MT)--一种保护生物体免受重金属伤害的金属结合蛋白--的mRNA表达增幅远低于镉和甲基汞。此外，我们还发现，在含有10−10和10−8M的水中，金鱼的血浆钙和降钙素(降钙素)水平升高。目前的数据首次证明，在硬骨鱼中，TBTA在不影响破骨活性的情况下抑制成骨细胞的活动，并扰乱金鱼的钙代谢，包括促钙激素。
To examine the direct effects of tributyltin acetate (TBTA) on osteoclasts and osteoblasts, teleost scale, which has both osteoclasts and osteoblasts and is similar to mammalian membrane bone, was used in the present study. The activities of tartrate-resistant acid phosphatase and alkaline-phosphatase, as respective indicators of activity in both cells, were used. In freshwater teleost (goldfish) and marine teleosts (nibbler and wrasse), the osteoclastic activity in the scales did not change as a result of TBTA treatment (10−9to 10−5M). However, the osteoblastic activity decreased in the goldfish, nibbler, and wrasse after 6 h of incubation. In goldfish, even 10−10M of TBTA significantly inhibited the osteoblastic activity. The inhibitory activity in goldfish was stronger than that in nibbler and wrasse. Therefore, details of the mechanism were examined using goldfish. The mRNA expressions of the estrogen receptor and insulin-like growth factor-I, which participate in osteoblastic growth and differentiation, decreased in the TBTA-treated scales. However, the mRNA expression of metallothionein (MT), a metal-binding protein that protects the organism from heavy metal, increased much less than those of cadmium and methyl-mercury. Furthermore, we showed that the plasma calcium and hypocalcemic hormone (calcitonin) level increased in goldfish kept in water containing TBTA (10−10and 10−8M). The current data are the first to demonstrate that, in teleosts, TBTA inhibits osteoblastic activity without affecting osteoclastic activity and disrupts the calcium metabolism, including the calcemic hormone, in goldfish.