Protective Effects of Sheng-Mai-San on Right Ventricular Dysfunction during Chronic Intermittent Hypoxia in Mice.
Protective Effects of Sheng-Mai-San on Right Ventricular Dysfunction during Chronic Intermittent Hypoxia in Mice.
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DOI：
10.1155/2016/4682786
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Yu BY
中科院分区：
文献类型：
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作者：
Chai CZ;Mo WL;Zhuang XF;Kou JP;Yan YQ;Yu BY
右心室（RV）功能障碍和衰竭导致心血管疾病的发病率和死亡率不断增加;然而，目前的治疗策略严重不足。生脉散用于治疗心脏病已有数百年的历史，但其对RV的保护作用尚未见报道。本研究旨在探讨丹参水提物对慢性间歇性缺氧（CIH）小鼠右室功能障碍的保护作用。结果表明，CIH小鼠模型在28天后出现RV功能障碍和适应不良代偿，SMS治疗可显著逆转这些变化。右室舒张功能恢复，收缩功能障碍减轻，包括右室每搏输出量和短轴缩短率增加，以及肺循环。结构上，SMS治疗抑制RV扩张，心肌细胞空泡化，超微结构异常，和裂解的caspase-3的表达。SMS通过降低RV中丙二醛（MDA）和4-羟基壬烯醛（4-HNE）的含量，提高超氧化物歧化酶（SOD）和血红素加氧酶-1（HO-1）的含量，抑制3-NT的过度表达，显示出显著的抗氧化活性。我们的研究结果表明，SMS保留RV的结构和功能，CIH暴露的小鼠参与调节活性氧和活性氮（RNS）的生产。
Right ventricular (RV) dysfunction and failure contribute to the increasing morbidity and mortality of cardiovascular diseases; however, current treatment strategies are grossly inadequate. Sheng-Mai-San (SMS) has been used to treat heart diseases for hundreds of years in China, and its protective effects on RV have not been observed. The present study was to investigate the protective effects of SMS aqueous extract on RV dysfunction in chronic intermittent hypoxia (CIH) mice model. The results showed that CIH mice model presented RV dysfunction and maladaptive compensation after 28-day-CIH and SMS treatment significantly reversed these changes. Diastolic function of RV was restored and systolic dysfunction was attenuated, including elevation of RV stroke volume and fractional shortening, as well as pulmonary circulation. Structurally, SMS treatment inhibited RV dilation, cardiomyocytes vacuolization, ultrastructure abnormalities, and the expression of cleaved caspase-3. Of importance, SMS showed remarkable antioxidant activity by decreasing the levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), increasing the levels of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1), as well as inhibiting the overexpression of 3-NT in RV. Our results indicate that SMS preserve RV structure and function in CIH-exposed mice by involving regulation in both ROS and Reactive Nitrogen Species (RNS) production.