Mussel-Inspired Coating of Polydopamine Directs Endothelial and Smooth Muscle Cell Fate for Re-endothelialization of Vascular Devices
Mussel-Inspired Coating of Polydopamine Directs Endothelial and Smooth Muscle Cell Fate for Re-endothelialization of Vascular Devices
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受贻贝启发的聚多巴胺涂层可指导内皮细胞和平滑肌细胞的命运，以实现血管装置的再内皮化
DOI：
10.1002/adhm.201200073
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发表时间：
2012-09-01
影响因子：
10
通讯作者：
Huang, Nan
中科院分区：
文献类型：
--
作者：
Yang, Zhilu;Tu, Qiufen;Huang, Nan
聚多巴胺（PDAM），贻贝粘附蛋白启发涂层，可以很容易地沉积到广泛的金属，无机和有机材料，也获得了在生物材料领域的兴趣。在这项工作中，PDAM作为涂层施加在316 L不锈钢（SS）支架上，并测试血管壁细胞、人脐静脉内皮细胞（HUVEC）和人脐动脉平滑肌细胞（HUASMC）作为再内皮化的预测因子的反应。研究发现，PDAM修饰的表面显著增强HUVEC的粘附、增殖和迁移、一氧化氮（NO）的释放和前列腺素I-2（PGI（2））的分泌。此外，PDAM修饰的表面显示出显著降低HUASMCs粘附和增殖的能力。作为血液接触材料，与基底316 L SS相比，PDAM倾向于改善血液相容性。值得注意的是，PDAM涂层显示出对支架压缩和扩张的变形行为的良好抵抗性。这些数据表明，PDAM作为血管支架或移植物中应用的血液接触材料的潜力。
Polydopamine (PDAM), a mussel adhesive protein inspired coating that can be easily deposited onto a wide range of metallic, inorganic, and organic materials, gains interest also in the field of biomaterials. In this work, PDAM is applied as coating on 316L stainless steel (SS) stents and the response of cells of the blood vessel wall, human umbilical vein endothelial cell (HUVEC), and human umbilical artery smooth muscle cell (HUASMC) as predictors for re-endothelialization is tested. It is found that the PDAM-modified surface significantly enhances HUVEC adhesion, proliferation, and migration, release of nitric oxide (NO), and secretion of prostaglandin I-2 (PGI(2)). Additionally, the PDAM-modified surface shows a remarkable ability to decrease the adhesion and proliferation of HUASMCs. As a blood-contacting material, the PDAM tends to improve the hemocompatibility compared with the substrate 316L SS. It is noteworthy that the PDAM coating shows good resistance to the deformation behavior of compression and expansion of a stent. These data suggest the potential of PDAM as a blood-contacting material for the application in vascular stents or grafts.