Capillary Liquid Chromatography at Ultrahigh Pressures
Capillary Liquid Chromatography at Ultrahigh Pressures
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DOI：
10.1146/annurev.anchem.1.031207.113014
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发表时间：
2010-01-01
期刊：
影响因子：
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通讯作者：
Jorgenson, James W.
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文献类型：
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作者：
Jorgenson, James W.
超高压液相色谱(UHPLC)是一种利用小于2微米的颗粒填充到25到100厘米长的毛细管柱中的液相色谱方法。这种长度的柱填充了这种细小的颗粒，需要在1000到7000巴(15000到100000磅/平方英寸)的压力下运行。这种技术的优势是高分离能力(理论板数从100,000到300,000)和运行时间从几分钟(等规线)到几个小时(长梯度)。本文讨论了超高效液相色谱的背景和理论基础、超高效液相硬件的实际应用、分离实例、未来的研究领域以及与超高效液相色谱竞争和互补的技术。
Ultrahigh-pressure liquid chromatography (UHPLC) is a method of liquid chromatography utilizing sub-2-mu m particles packed into capillary columns 25 to 100 cm long. Columns of this length packed with particles this fine require operation with pressures from 1,000 to 7,000 bar (15,000 to 100,000 psi). The advantages of this technique are high separation powers (theoretical plate counts from 100,000 to 300,000) and run times from a few minutes (isocratic) to a few hours (long gradients). This review discusses the background and theoretical basis of UHPLC, practical aspects of UHPLC hardware, examples of separations, future areas for research in UHPLC, and techniques that are both competitive with and complementary to UHPLC.