EXCLUSION OF SUPERINFECTING HOMOLOGOUS VIRUS BY SINDBIS VIRUS-INFECTED AEDES-ALBOPICTUS (MOSQUITO) CELLS
EXCLUSION OF SUPERINFECTING HOMOLOGOUS VIRUS BY SINDBIS VIRUS-INFECTED AEDES-ALBOPICTUS (MOSQUITO) CELLS
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DOI：
10.1128/jvi.58.1.81-86.1986
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发表时间：
1986-04-01
影响因子：
5.4
通讯作者：
BROWN, DT
中科院分区：
文献类型：
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作者：
CONDREAY, LD;BROWN, DT
组织培养的白纹伊蚊（蚊子）细胞被甲病毒感染最终导致持续感染的细胞群，其可以在实验室中维持多年。这种培养物的一个特征是它不支持重复感染的同源病毒的复制。我们以前已经证明，持续感染辛德毕斯病毒的蚊子细胞产生一种抗病毒剂，当应用于未感染的蚊子细胞时，这种抗病毒剂会抑制辛德毕斯病毒的复制。在抗病毒剂处理的细胞中排除病毒复制类似于在甲病毒感染的脊椎动物细胞中描述的同源干扰现象。在这项研究中，我们研究了hmdr3干扰在三种蚊细胞系中的表达，并比较了同源干扰的表达与抗病毒活性的影响。发现细胞系表达同源干扰的能力不同，有证据表明蚊子细胞可能通过同源干扰或通过抗病毒剂的作用抑制复制。
The infection of tissue-cultured Aedes albopictus (mosquito) cells by an alphavirus ultimately results in a persistently infected cell population which can be maintained in the laboratory for years. One characteristic of this culture is that it will not support the replication of superinfecting homologous virus. We have previously shown that mosquito cells persistently infected with Sindbis virus produce an antiviral agent which when applied to uninfected mosquito cells suppresses Sindbis virus replication. The exclusion of virus replication in the antiviral-agent-treated cells is similar to the phenomenon of homologous interference described in alphavirus-infected vertebrate cells. In this study we examined the expression of hmologous interference in three lines of mosquito cells and compared the expression of homologous interference to the effects of the antiviral activity. The cell lines were found to differ in their ability to express homologous interference, and evidence suggests that the mosquito cells may suppress replication by homologous interference or by the action of the antiviral agent.