Cyclohexane, naphthalene, and diesel fuel increase oxidative stress, CYP153, sodA, and recA gene expression in Rhodococcus erythropolis
Cyclohexane, naphthalene, and diesel fuel increase oxidative stress, CYP153, sodA, and recA gene expression in Rhodococcus erythropolis
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DOI：
10.1002/mbo3.855
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发表时间：
2019-09-01
期刊：
影响因子：
3.4
通讯作者：
Sazykina, Marina
中科院分区：
文献类型：
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作者：
Sazykin, Ivan;Makarenko, Maksim;Sazykina, Marina
在本研究中，我们比较了环己烷、萘和柴油存在下碳氢化合物降解红平红球菌中 CYP153、sodA、sodC 和 recA 基因的表达和 ROS 生成。添加所有研究的碳氢化合物后，细胞色素 P450、sodA（编码 Fe/Mn 超氧化物歧化酶）、recA 和超氧阴离子自由基生成率的表达均有所增加。在萘存在的情况下记录了 CYP153、sodA 和 recA 基因表达的峰值。相同的底物上调 Cu/Zn 超氧化物歧化酶基因 sodC。环己烷产生最高水平的超氧阴离子自由基。过氧化氢在富含柴油的介质中积聚。总而言之，碳氢化合物生物转化导致氧化应激以及抗氧化酶和 CYP153 基因的上调，并增加红平红细胞中的 DNA 修复水平。
In this study, we compared the expression of CYP153, sodA, sodC, and recA genes and ROS generation in hydrocarbon-degrading Rhodococcus erythropolis in the presence of cyclohexane, naphthalene, and diesel fuel. The expression of cytochrome P450, sodA (encoding Fe/Mn superoxide dismutase), recA, and superoxide anion radical generation rate increased after the addition of all studied hydrocarbons. The peak of CYP153, sodA, and recA gene expression was registered in the presence of naphthalene. The same substrate upregulated the Cu/Zn superoxide dismutase gene, sodC. Cyclohexane generated the highest level of superoxide anion radical production. Hydrogen peroxide accumulated in the medium enriched with diesel fuel. Taken together, hydrocarbon biotransformation leads to oxidative stress and upregulation of antioxidant enzymes and CYP153 genes, and increases DNA reparation levels in R. erythropolis cells.