Genomic Exploration of the Hemiascomycetous Yeasts:: 5.: Saccharomyces bayanus var. uvarum

Genomic Exploration of the Hemiascomycetous Yeasts:: 5.: Saccharomyces bayanus var. uvarum
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DOI:
10.1016/s0014-5793(00)02276-6
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发表时间:
2000-12-22
期刊:
影响因子:
3.5
通讯作者:
Durrens, P
Durrens, P
中科院分区:
生物学3区
文献类型:
--
作者:
Bon, E;Neuvéglise, C;Durrens, P

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比亚努斯酵母变种这里研究的 uvarum 是最接近酿酒酵母的物种。随机序列标签 (RST) 使我们能够识别酿酒酵母中 2789 个开放阅读框 (ORF) 的同源物、在酿酒酵母中重复的 ORF、但在酿酒酵母中没有的 ORF、着丝粒、tRNA、Ty1/2 和 Ty4 逆转录转座子的同源物以及完整的 rDNA 重复序列。只有 13 个 RST 似乎与其他生物体中的序列同源,但与酿酒酵母中的序列不同。由于两个物种之间的同线性非常高,失去同线性的情况表明基因组进化的特殊机制。相应的RST显示S,uvarum可以在没有任何S,cervisiae DNA渗入的情况下存在,EMBL数据库中的登录号为AL397139至AL402278,(C)2000欧洲生化协会联合会。由 Elsevier Science B.V. 出版。保留所有权利。
Saccharomyces byanus var. uvarum investigated here is the species closest to Saccharomyces cerevisiae . Random sequence tags (RSTs) allowed us to identify homologues to 2789 open reading frames (ORFs) in S. cervisiae, ORFs duplicated in S. uvarum but not in S.cervisiae, centromeres, tRNAs, homologues of Ty1/2 and Ty4 retrotransposons, and a complete rDNA repeat. Only 13 RSTs seem to be homologous to sequences in other organisms but not in S.cervisiae. As the synteny between the two species is very high, cases in which synteny is lost suggest special mechanisms of genome evolution. The corresponding RSTs revealed that S, uvarum can exist without any S, cervisiae DNA introgression, Accession numbers are from AL397139 to AL402278 in the EMBL databank, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.