Nitric oxide-dependent proton translocation in various denitrifiers
Nitric oxide-dependent proton translocation in various denitrifiers
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DOI：
10.1128/jb.163.3.837-840.1985
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发表时间：
1985-09
影响因子：
3.2
通讯作者：
J. Shapleigh;W. Payne
中科院分区：
文献类型：
--
作者：
J. Shapleigh;W. Payne
呼吸NO导致短暂的质子易位在厌氧生长的细胞的四个生理上不同的细菌。类球红球藻（Rhodopulus sphaeroides subsp.）固氮菌，“无色杆菌cycloclastes”，和根瘤菌，分别给出了3.65，4.96，1.94，和1.12的H+/NO比。抗霉素A可完全抑制P. acetificans中NO依赖的质子转运，严重抑制R. sphaeroides菌株在NO呼吸与抗霉素A抑制细胞提供了人工电子源质子的周质消耗的证据。获得的值与用于将NO还原成N2 O的0.5 mol H+/mol NO和用于将NO还原成N2的1.0 mol H+/mol NO的预期比率一致。这些数据是一致的存在下，一个独特的NO还原酶发现，只有在厌氧生长的微生物细胞。
Respiration of NO resulted in transient proton translocation in anaerobically grown cells of four physiologically diverse denitrifiers. Paracoccus denitrificans, Rhodopseudomonas sphaeroides subsp. denitrificans, "Achromobacter cycloclastes," and Rhizobium japonicum gave, respectively, H+/NO ratios of 3.65, 4.96, 1.94, and 1.12. Antimycin A completely inhibited NO-dependent proton translocation in P. denitrificans and severely restricted translocation in the R. sphaeroides strain. Proton uptake during NO respiration with antimycin A-inhibited cells supplied with an artificial electron source provided evidence for the periplasmic consumption of protons. Values obtained were consistent with the expected ratios of 0.5 mol of H+/mol of NO for reduction of NO to N2O and 1.0 mol of H+/mol of NO for reduction of NO to N2. These data are consistent with the presence of a unique NO reductase found only in anaerobically grown denitrifying cells.