Visualization of degradation of injectable thermosensitive hydroxypropyl chitin modified by aggregation-induced emission
Visualization of degradation of injectable thermosensitive hydroxypropyl chitin modified by aggregation-induced emission
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聚集诱导发射修饰的可注射热敏羟丙基甲壳素降解的可视化
DOI：
10.1016/j.carbpol.2022.119739
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Xulin Jiang
中科院分区：
文献类型：
--
作者：
Long Huang;Hejun Zhan;Xulin Jiang
可逆温敏性羟丙基甲壳素（HPCH）在药物载体、组织工程、创伤修复和抗菌止血等方面有着广泛的应用。为了实现对羟丙基甲壳素（HPCH）在体内降解过程的非侵入性和实时可视化，在水溶液中将聚集诱导发射荧光团（AIEgen）共价引入HPCH中，制备了温敏荧光羟丙基甲壳素（FHPCH）。所得荧光FHPCH水凝胶在紫外光照射下显示出强烈的黄绿色荧光特征，使得水凝胶具有可逆的热敏性和可靠的注射性。FHPCH水凝胶的体外酶降解研究表明，荧光强度的衰减与水凝胶的重量损失很好地匹配。通过26号针头注射小鼠进行实时荧光成像确实表明，AIE修饰的可注射热敏FHPCH可用于体内降解的非侵入性、连续和实时可视化和定量分析。这为类似植入材料的无创实时监测提供了一种新方法。
The reversible thermosensitive hydroxypropyl chitin (HPCH) has been widely investigated for drug delivery, tissue engineering, wound repair and antibacterial hemostasis. To achieve non-invasive and real-time visualization of in vivo degradation of HPCH, aggregation-induced emission fluorogen (AIEgen) was introduced covalently in HPCH in water to obtain thermosensitive fluorescent hydroxypropyl chitin (FHPCH) avoiding using any organic solvent. The obtained fluorescent FHPCH hydrogel showed strong yellow-green fluorescence feature under UV irradiation, enabling visualization of the hydrogel with reversible thermosensitivity and reliable injectability. The in vitro enzymatic degradation study of FHPCH hydrogel showed that the attenuation of fluorescence intensity well matched with hydrogel weight loss. The injection through a 26-gauge needle for real-time fluorescence imaging in mice indeed indicated that AIE modified injectable thermosensitive FHPCH can be used for non-invasive, continuous and real-time visualization and quantitative analysis of in vivo degradation. This provides a new method for non-invasive real-time monitoring of similar implantable materials.