Simultaneous determination of 17 antiretroviral drugs in human plasma for quantitative analysis with liquid chromatography-tandem mass spectrometry
Simultaneous determination of 17 antiretroviral drugs in human plasma for quantitative analysis with liquid chromatography-tandem mass spectrometry
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DOI：
10.1002/bmc.865
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发表时间：
2007-10-01
影响因子：
1.8
通讯作者：
Kashuba, Angela D. M.
中科院分区：
文献类型：
--
作者：
Jung, Byung Hwa;Rezk, Naser L.;Kashuba, Angela D. M.
建立了同时测定L血浆中17种抗逆转录病毒药物的灵敏方法，包括核苷类似物逆转录酶抑制剂、非核苷类似物逆转录酶抑制剂、蛋白酶抑制剂和核苷酸类似物逆转录酶抑制剂。该方法采用高效液相色谱-串联质谱仪-电喷雾电离。在多反应监测模式下对分析物进行监测，一次进样后将极性从正到负切换到正，以检测所有化合物。采用液-液萃取和蛋白质沉淀相结合的方法提取所有化合物，所有分析物的回收率至少为75%。奈非那韦、吲哚那韦和阿巴卡韦的日内和日间精密度均在1-500 ng/mL之间，地丹苷和司他夫定的日内和日间精密度均在10-500 ng/mL之间，其他化合物的日内和日间精密度均在5-500 ng/mL之间。该方法适用于临床样本有限(L 50亩)的抗逆转录病毒药物定量和筛选。版权所有(C)2007 John Wiley&Sons，Ltd.
This work describes a sensitive method for the simultaneous determination of 17 antiretroviral drugs including nucleoside analog reverse transcriptase inhibitors, non-nucleoside analog reverse transcriptase inhibitors, protease inhibitors and a nucleotide analog reverse transcriptase inhibitor in 50 mu L of human plasma. This method employed high-performance liquid chromatography-tandem mass spectrometry with electrospray ionization. The analytes were monitored in multiple reaction monitoring mode and the polarity was switched from positive to negative to positive to detect all compounds after a single injection. A combination of liquid-liquid extraction and protein precipitation was used to extract all compounds, with at least 75% recovery for all analytes. Within- and between-day accuracies were at least 85% for 1-500 ng/mL for nelfinavir, indinavir and abacavir, 10-500 ng/mL for didanosine and stavudine and 5-500 ng/mL for all other compounds. This method is very effective for quantifying and screening antiretroviral drugs in clinical samples with limited (50 mu L) volumes. Copyright (c) 2007 John Wiley & Sons, Ltd.