Serial dilution via surface energy trap-assisted magnetic droplet manipulation.
Serial dilution via surface energy trap-assisted magnetic droplet manipulation.
复制标题
通过表面能陷阱辅助磁滴操作进行连续稀释。
DOI：
10.1039/c3lc50915j
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发表时间：
2013
期刊：
影响因子：
6.1
通讯作者：
Wang,Tza-Huei
中科院分区：
文献类型：
--
作者：
Zhang,Yi;Shin,DongJin;Wang,Tza-Huei
本文演示了一种在开放表面平台上以液滴形式生成精确连续稀释的简便方法。该方法依赖于表面能陷阱 (SET) 的使用，即在聚四氟乙烯涂层玻璃基板上蚀刻高表面能区域，以协助磁力操纵液滴，以计量和分配规定体积的液体，以制备连续稀释液。分配液体的体积可以通过 SET 的尺寸精确控制，有利于生成高线性度的浓度分布。我们已将这种方法应用于抗生素连续稀释液的生成，以进行抗微生物敏感性测试 (AST)。
This paper demonstrates a facile method of generating precise serial dilutions in the form of droplets on an open surface platform. The method relies on the use of surface energy traps (SETs), etched areas of high surface energy on a Teflon coated glass substrate, to assist in the magnetic manipulation of droplets to meter and dispense liquid of defined volumes for the preparation of serial dilutions. The volume of the dispensed liquid can be precisely controlled by the size of the SETs, facilitating generation of concentration profiles of high linearity. We have applied this approach to the generation of serial dilutions of antibiotics for anti-microbial susceptibility testing (AST).