Structural Identification of Novel Oligosaccharides Produced by Lactobacillus bulgaricus and Lactobacillus plantarum
Structural Identification of Novel Oligosaccharides Produced by Lactobacillus bulgaricus and Lactobacillus plantarum
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保加利亚乳杆菌和植物乳杆菌产生的新型低聚糖的结构鉴定
DOI：
10.1021/jf300917m
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发表时间：
2012-05-16
影响因子：
6.1
通讯作者：
Gaenzle, Michael G.
中科院分区：
文献类型：
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作者：
Black, Brenna A.;Lee, Vivian S. Y.;Gaenzle, Michael G.
当存在合适的受体碳水化合物时，乳酸菌的β-半乳糖苷酶（β-Gal）从乳糖产生寡糖。本研究旨在阐明N-乙酰氨基葡萄糖（GlcNAc）和岩藻糖的半乳糖基化形成的寡糖的结构。保加利亚乳杆菌的粗细胞提取物和植物乳杆菌的LacLM用作β-Gal活性的来源。通过液相色谱-串联质谱法（LC-MS/MS）和与外标物的比较，将通过GlcNAc的半乳糖基化获得的二糖鉴定为Gal-beta-（1 -> 4）-GlcNAc或Gal-beta-（1 -> 6）-GlcNAc。通过LC-MS、所选源内碎片离子的MS/MS光谱分析及其相对保留时间，将三糖鉴定为Gal-β-（1 -> 6）-Gal-β-（1 ->[4或6]）-GlcNAc。LC-MS分析显示存在五种半乳糖基化岩藻糖苷，但无法鉴定其连接类型，部分原因是缺乏参比化合物。乳酸菌的β-Gal可作为治疗性寡糖的化学酶促合成的合适工具。
beta-Galactosidases (beta-Gal) of lactic acid bacteria produce oligosaccharides from lactose when suitable acceptor carbohydrates are present. This study aimed to elucidate the structure of oligosaccharides formed by galactosylation of N-acetylglucosamine (GlcNAc) and fucose. Crude cellular extract of Lactobacillus bulgaricus and LacLM of Lactobacillus plantarum were used as sources of beta-Gal activity. Disaccharides obtained by galactosylation of GlcNAc were identified as Gal-beta-(1 -> 4)-GlcNAc or Gal-beta-(1 -> 6)-GlcNAc by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and comparison with external standards. Trisaccharides were identified as Gal-beta-(1 -> 6)-Gal-beta-(1 ->[4 or 6])-GlcNAc by LC-MS, analysis of the MS/MS spectra of selected in-source fragment ions, and their relative retention times. LC-MS analysis revealed the presence of five galactosylated fucosides, but their linkage type could not be identified, partly due to the lack of reference compounds. beta-Gal of lactic acid bacteria may serve as suitable tools for the chemoenzymatic synthesis of therapeutic oligosaccharides.