Near-infrared fluorescence imaging platform for quantifying in vivo nanoparticle diffusion from drug loaded implants.
Near-infrared fluorescence imaging platform for quantifying in vivo nanoparticle diffusion from drug loaded implants.
复制标题
近红外荧光成像平台，用于量化体内纳米颗粒从药物载荷植入物扩散。
DOI：
10.2147/ijn.s93324
复制
发表时间：
2016
影响因子：
8
通讯作者：
Niedre M
中科院分区：
文献类型：
--
作者：
Markovic S;Belz J;Kumar R;Cormack RA;Sridhar S;Niedre M
Drug loaded implants are a new, versatile technology platform to deliver a localized payload of drugs for various disease models. One example is the implantable nanoplatform for chemo-radiation therapy where inert brachytherapy spacers are replaced by spacers doped with nanoparticles (NPs) loaded with chemotherapeutics and placed directly at the disease site for long-term localized drug delivery. However, it is difficult to directly validate and optimize the diffusion of these doped NPs in in vivo systems. To better study this drug release and diffusion, we developed a custom macroscopic fluorescence imaging system to visualize and quantify fluorescent NP diffusion from spacers in vivo. To validate the platform, we studied the release of free fluorophores, and 30 nm and 200 nm NPs conjugated with the same fluorophores as a model drug, in agar gel phantoms in vitro and in mice in vivo. Our data verified that the diffusion volume was NP size-dependent in all cases. Our near-infrared imaging system provides a method by which NP diffusion from implantable nanoplatform for chemo-radiation therapy spacers can be systematically optimized (eg, particle size or charge) thereby improving treatment efficacy of the platform.
