Localization in the vagal ganglia of calcitonin gene-related peptide- and calretinin-immunoreactive neurons that innervate the cervical and the subdiaphragmatic esophagus of the rat
Localization in the vagal ganglia of calcitonin gene-related peptide- and calretinin-immunoreactive neurons that innervate the cervical and the subdiaphragmatic esophagus of the rat
复制标题
DOI：
10.1016/j.jchemneu.2011.10.004
复制
发表时间：
2012-01-01
影响因子：
2.8
通讯作者：
Seki, Makoto
中科院分区：
文献类型：
--
作者：
Hayakawa, Tetsu;Kuwahara-Otani, Sachi;Seki, Makoto
我们已经确定了降钙素基因相关肽免疫反应（CGRP-IR）和calretininin-IR神经元在迷走神经节的神经支配的颈部或颈下食管的本地化。许多CGRP免疫反应神经元被发现只在位于颅腔的颈静脉神经节。Calretinin-ir神经元分布于整个迷走神经节。将荧光金注入颈段食管，在颈静脉神经节和结状神经节内出现大量荧光金标记神经元。将荧光金注入食管下段，在结状神经节内出现大量荧光金标记神经元。在颈段食管注射荧光金的情况下，免疫组织化学和逆行追踪相结合的双标记显示，约40%的荧光金标记神经元在颈静脉神经节表达CGRP样免疫反应，约20%的荧光金标记神经元在颈静脉和结状神经节均表达calretinin样免疫反应。在注射到食管下的情况下，只有少数荧光金标记的神经元表达CGRP样免疫反应性或calretinin样免疫反应性的迷走神经节。这些结果表明，颈段食管接受来自颈静脉神经节中的许多CGRP免疫阳性神经元和来自颈静脉和结状神经节中的calretinin-ir神经元的投射，而颈下段食管仅接受来自迷走神经节中的少数CGRP免疫阳性神经元和calretin-ir神经元的投射。(C)2011爱思唯尔有限公司版权所有。
We have determined the localization of calcitonin gene-related peptide-immunoreactive (CGRP-ir) and calretinin-ir neurons in the vagal ganglia that innervate the cervical or subdiaphragmatic esophagus. Many CGRP-ir neurons were found exclusively in the jugular ganglion located in the cranial cavity. Calretinin-ir neurons were distributed throughout the vagal ganglia. Injection of Fluorogold into the cervical esophagus resulted in many Fluorogold-labeled neurons in the jugular and nodose ganglia. Injection of Fluorogold into the subdiaphragmatic esophagus resulted in many Fluorogold-labeled neurons, with most in the nodose ganglion. In the case of Fluorogold injection into the cervical esophagus, double-labeling combining immunohistochemistry and retrograde tracing showed that about 40% of the Fluorogold-labeled neurons in the jugular ganglion express CGRP-like immunoreactivity, and about 20% of the Fluorogold-labeled neurons in both the jugular and nodose ganglia express calretinin-like immunoreactivity. In the case of injection into the subdiaphragmatic esophagus, only a few Fluorogold-labeled neurons express CGRP-like immunoreactivity or calretinin-like immunoreactivity in the vagal ganglia. These results indicate that the cervical esophagus receives projections from many CGRP-ir neurons in the jugular ganglion and from calretinin-ir neurons in the jugular and nodose ganglia, while the subdiaphragmatic esophagus receives projections from only a few CGRP-ir and calretinin-ir neurons in the vagal ganglia. (C) 2011 Elsevier B.V. All rights reserved.