The Amino Acid Sequence of Ascorbate Peroxidase from Tea Has a High Degree of Homology to That of Cytochrome c Peroxidase from Yeast
The Amino Acid Sequence of Ascorbate Peroxidase from Tea Has a High Degree of Homology to That of Cytochrome c Peroxidase from Yeast
复制标题
茶叶抗坏血酸过氧化物酶的氨基酸序列与酵母细胞色素c过氧化物酶具有高度同源性
DOI：
10.1093/oxfordjournals.pcp.a078228
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发表时间：
1992
影响因子：
4.9
通讯作者：
K. Asada
中科院分区：
文献类型：
--
作者：
Gong;S. Sano;K. Asada
用赖氨酰内肽酶对茶树叶片抗坏血酸过氧化物酶同工酶进行酶切，并对酶切片段的氨基酸序列进行了测定。这些序列占整个蛋白质中氨基酸的64%。其中一个肽的序列可以与包括近端组氨酸的区域进行比对，该组氨酸在愈创木酚过氧化物酶和细胞色素过氧化物酶中充当血红素铁的第五配体。来自抗坏血酸过氧化物酶的肽序列与来自酵母的细胞色素过氧化物酶的序列比与来自植物的愈创木酚过氧化物酶的序列具有更高程度的同源性。此外，三个抗坏血酸过氧化物酶的肽显示出高度的同源性，磷酸丙糖异构酶从玉米。从现有的氨基酸序列和酶和抗坏血酸和cytochromecperoxidases的分子特性，我们建议，这些过氧化氢清除过氧化物酶，使用cytochromecor抗坏血酸作为电子供体来自相同的祖先蛋白质。
The chloroplast isozyme of ascorbate peroxidase from tea leaves was digested with lysyl endopeptidase, and the amino acid sequences of the peptide fragments were determined. These sequences accounted for 64% of the amino acids in the entire protein. The sequence of one of the peptides can be aligned with the region which includes the proximal histidine that serves as the fifth ligand of the heme iron in guaiacol peroxidases and cytochromecperoxidase. The sequences of the peptides from ascorbate peroxidase exhibit a higher degree of homology to the sequence of cytochromecperoxidase from yeast than to those of guaiacol peroxidases from plants. In addition, three of the peptides from ascorbate peroxidase show a high degree of homology to triose-phosphate isomerase from maize. From the available amino acid sequences and the enzymatic and molecular properties of ascorbate and cytochromecperoxidases, we propose that these hydrogen peroxide-scavenging peroxidases that use either cytochromecor ascorbate as the electron donor originated from the same ancestral protein.