Birth of normal offspring from mouse eggs activated by a phospholipase Cζ protein lacking three EF-hand domains
Birth of normal offspring from mouse eggs activated by a phospholipase Cζ protein lacking three EF-hand domains
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DOI：
10.1262/jrd.19168
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发表时间：
2008-08-01
影响因子：
1.8
通讯作者：
Baba, Tadashi
中科院分区：
文献类型：
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作者：
Nakanishi, Tomoko;Ishibashi, Naoko;Baba, Tadashi
精子特异性磷脂酶C （PLC zeta）是一种候选的Ca2+振荡诱导因子，在精子与卵子融合时被引入卵浆。除了全长647个残基的PLC zeta外，已知在小鼠睾丸中还存在缺乏n端110个氨基酸的s-PLC zeta。在这项研究中，我们试图通过注射圆形精子获得s-PLC zeta活化卵子的足月子代。中期捕获的卵注射高浓度的s-PLC zeta RNA后正常发育成囊胚。将圆形精细胞核注入经s-PLC zeta RNA激活的末期ii期卵中，将发育好的4细胞胚胎移植到假妊娠小鼠体内，成功获得3个活子代。这三个后代都长成了正常的成年鼠，并繁殖出健康的第二代小鼠。
Sperm-specific phospholipase C, PLC zeta, is a candidate for the Ca2+ oscillation-inducing factor that is introduced into the ooplasm upon sperm-egg fusion. In addition to the 647-residue full-length PLC zeta, s-PLC zeta lacking the N-terminal 110 amino acids is known to be present in the mouse testis. In this study, we attempted to obtain fullterm offspring from s-PLC zeta-activated eggs by round spermatid injection. Metaphase II-arrested eggs injected with a high RNA concentration of s-PLC zeta RNA normally developed to blastocysts. When the round spermatid nucleus was injected into telophase II-stage eggs previously activated by s-PLC zeta RNA, three live offspring were successfully obtained by transfer of the developed 4-cell embryos to pseudopregnant mice. These three offspring all grew to be normal adults and reproduced healthy second-generation mice.