Comparison of the Expression of Periodontal Markers in Dental and Bone Marrow-derived Mesenchymal Stem Cells
Comparison of the Expression of Periodontal Markers in Dental and Bone Marrow-derived Mesenchymal Stem Cells
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DOI：
10.2174/1874210602014010196
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发表时间：
2020-01-01
影响因子：
1.3
通讯作者：
Ghuman, Mandeep
中科院分区：
文献类型：
--
作者：
Garna, Devy;Kaur, Manmeet;Ghuman, Mandeep
背景：牙周膜干细胞是间充质干细胞的来源，但目前尚不清楚它们的表型是否与来自不同组织的间充质干细胞不同，例如来自骨髓的间充质干细胞。目的：研究牙周膜干细胞在组成型和成骨分化过程中与骨髓源性间充质干细胞和牙髓干细胞相比，牙周膜干细胞、骨髓源性间充质干细胞、牙髓干细胞和成骨细胞的假定PDL标志物asporin、periostin、nestin和牙骨质蛋白1的表达。通过qRT-PCR和免疫荧光染色检测成骨诱导过程中牙周标志物相关基因的表达。结果：在牙周膜干细胞中检测到Asporin表达，并且在培养期间显著增加（在诱导后21天上调x53倍）。在成骨分化过程中，牙周膜细胞中asporin的表达显著降低，而牙髓细胞中periostin的表达显著降低。成骨细胞中不存在骨膜蛋白表达，但在培养的所有其他细胞中，随着时间的推移，骨膜蛋白表达逐渐增加。巢蛋白主要表达于牙周膜和牙髓细胞，成骨细胞和骨髓细胞中基本不表达。牙骨质蛋白-1在成骨诱导后的骨髓细胞和成骨细胞中表达最高。结论：该结果进一步证明了牙周韧带来源的间充质干细胞和骨髓来源的间充质干细胞在表型上是不同的。牙周膜标记物也在牙髓干细胞中表达。
Background:Periodontal ligament stem cells are a source of mesenchymal stem cells, but it is unclear whether their phenotype is distinct from mesenchymal stem cells derived from different tissues, such as those derived from bone marrow.Objective:To investigate the expression of the putative PDL markers asporin, periostin, nestin and cementum protein 1, by periodontal ligament stem cells both constitutively and during osteogenic differentiation when compared to bone marrow-derived mesenchymal stem cells, and dental pulp stem cells.Methods:The primary human periodontal ligament, bone marrow, and dental pulp stem cells, and osteoblasts from different donors were cultured in vitro. The expression of periodontal marker associated genes during osteogenic induction was tested by qRT-PCR and immunofluorescence staining.Results:Asporin expression was detected in periodontal ligament stem cells and increased markedly during the time in culture (upregulated x53 fold at 21 days post-induction). During osteogenic differentiation, asporin expression significantly decreased in periodontal ligament cells whereas periostin significantly decreased in dental pulp cells. Periostin expression was absent in osteoblasts, but expression gradually increased in all other cells with time in culture. Nestin expression was mainly seen in the periodontal ligament and dental pulp cells and was largely absent in osteoblasts and bone marrow cells. Cementum protein-1 was most highly expressed in bone marrow cells and osteoblasts following osteogenic induction.Conclusions:The results provide further evidence that periodontal ligament-derived and bone marrow derived mesenchymal stem cells are phenotypically distinct. Periodontal markers are also expressed in dental pulp stem cells.