HETEROGENEOUS EXPRESSION OF CD23 EPITOPES BY EOSINOPHILS FROM PATIENTS - RELATIONSHIPS WITH IGE-MEDIATED FUNCTIONS
HETEROGENEOUS EXPRESSION OF CD23 EPITOPES BY EOSINOPHILS FROM PATIENTS - RELATIONSHIPS WITH IGE-MEDIATED FUNCTIONS
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DOI：
10.1002/eji.1830211019
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发表时间：
1991-10-01
影响因子：
5.4
通讯作者：
CAPRON, A
中科院分区：
文献类型：
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作者：
CAPRON, M;TRUONG, MJ;CAPRON, A
人嗜酸性粒细胞上的IgE受体(Fc-epsilon-RII)与B细胞分化标志物CD23具有一些共同的特征。我们用流式细胞术检测了CD23的不同表位在纯化的嗜酸性粒细胞上的表达。骨髓瘤IgE蛋白的结合与针对B细胞CD23的IgE结合部位的单抗(MAb135)的结合有关。使用另外两种针对IgE结合位点的抗CD23单抗(8-30或3-5)，这些CD23表位在不同嗜酸性粒细胞上的低表达。用CD23的cDNA探针对嗜酸性粒细胞RNA进行Northern印迹分析，发现在六名表达膜CD23的患者中有三名患者有低丰度的转录本。所有抗CD23单抗对IgE介导的细胞毒作用和IgE与嗜酸性粒细胞结合的抑制作用清楚地表明CD23相关分子参与了IgE依赖的嗜酸性粒细胞功能。
The receptor for IgE (Fc-epsilon-RII) on human eosinophils presents some common characteristics with CD23, a differentiation marker of B cells. We have used flow cytometry for evaluating the expression of various epitopes of CD23 on purified eosinophils from patients with eosinophilia. A correlation was found between the binding of myeloma IgE protein and the binding of a monoclonal antibody (mAb 135), directed against the IgE-binding site of B cell CD23. Using two additional anti-CD23 mAb, directed (8-30) or not (3-5) against the IgE-binding site, a low expression of these CD23 epitopes was observed on eosinophils from different eosinophilic patients. Northern blot analysis of eosinophil RNA with the cDNA probe of CD23 revealed a low-abundancy transcript in three of the six patients expressing membrane CD23. The inhibition by all anti-CD23 mAb of IgE-mediated cytotoxicity and IgE binding to eosinophils clearly indicated the participation of a CD23-related molecule in IgE-dependent eosinophil functions.