Halofilum ochraceum gen. nov., sp nov., a gammaproteobacterium isolated from a marine solar saltern
Halofilum ochraceum gen. nov., sp nov., a gammaproteobacterium isolated from a marine solar saltern
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Halofilum ochraceum gen.
DOI：
10.1099/ijsem.0.001718
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发表时间：
2017
影响因子：
2.8
通讯作者：
Du Zong-Jun
中科院分区：
文献类型：
--
作者：
Xia Jun;Zhao Jin-Xin;Sang Jin;Chen Guan-Jun;Du Zong-Jun
从威海沿海的一个海洋太阳盐场中分离到一株革兰氏阴性、氧化物酶阴性、过氧化氢酶阳性的兼性厌氧菌，命名为XJ16T。菌株XJ16T的细胞较长，呈杆状。菌落呈赭色，能够将硝酸盐还原为亚硝酸盐。最佳生长条件为33-37 °C(范围20-45 °C)和8-10 %(w/v)氯化钠(范围2-20 %)。最适生长pH范围为6.5~9.5，最适生长pH为7.5~8.0。基于16S rRNA基因序列的系统发育分析表明，菌株XJ16T与变形杆菌属亲缘关系较近。菌株XJ16T的16SRRNA基因序列与硫代嗜硫杆菌HL-EbGr7T的16S rRNA基因序列有93.1 %的相似性，与脱氮硫碱性弧菌ALJDT的16S rRNA基因序列有93.0 %的相似性。基因组G+C含量为65.9Mol%( )。唯一的呼吸对苯二酚为Q-8，主要的细胞脂肪酸(>；10 %)为异C15 ： 02-OH/C16 ： 1ω7c、C18 ： 0和C16 ： 010-CH3。菌株XJ16T的主要极性脂类为二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和磷脂酰胆碱。根据这些系统发育和生理生化特征，XJ16T菌株应该被归类为一个新种，该新种属于海鞘螺科一个新属，命名为Halofilum ochraceumgen。Nov.，sp.11月是提议的。模式种的模式株为XJ16T(=KCTC 42259T=MCCC 1H00120T=CICC 23817T)。
A Gram-stain-negative, oxidase-negative, catalase-positive, facultative anaerobe, designated XJ16T, was isolated from a marine solar saltern on the coast of Weihai, China. Cells of strain XJ16Twere long and rod-shaped. The colonies were ochre in colour and were able to reduce nitrate to nitrite. Optimal growth occurred at 33–37 °C (range, 20–45 °C) and in the presence of 8–10 % (w/v) NaCl (range, 2–20 %). The pH range for growth was found to be 6.5–9.5, with optimum growth at pH 7.5–8.0. Phylogenetic analysis based on the 16S rRNA gene sequence demonstrated that strain XJ16Twas related to the phylumProteobacteria. The most closely related neighbours were species of the genusThioalkalivibrio, and the 16S rRNA gene sequence of strain XJ16Tshared 93.1 % similarity with that ofThioalkalivibrio sulfidiphilusHL-EbGr7Tand 93.0 % similarity with that ofThioalkalivibrio denitrificansALJDT. The G+C content of the genomic DNA was 65.9 mol% (HPLC). The sole respiratory quinone was Q-8, and the predominant cellular fatty acids (>10 %) were iso-C15 : 02-OH/C16 : 1ω7c, C18 : 0and C16 : 010-CH3. The predominant polar lipids in strain XJ16Twere diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. Based on these phylogenetic, physiological and biochemical characteristics, strain XJ16Tshould be classified representing a novel species of a new genus within the familyEctothiorhodospiraceae, for which the nameHalofilum ochraceumgen. nov., sp. nov. is proposed. The type strain of the type species is XJ16T(=KCTC 42259T=MCCC 1H00120T=CICC 23817T).