Imaging renal structures by X-ray phase-contrast microtomography
Imaging renal structures by X-ray phase-contrast microtomography
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DOI：
10.1038/ki.2009.42
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发表时间：
2009-05-01
影响因子：
19.6
通讯作者：
Akatsuka, Takao
中科院分区：
文献类型：
--
作者：
Wu, Jin;Takeda, Tohoru;Akatsuka, Takao
基于X射线晶体干涉仪的X射线相衬显微断层摄影（相衬显微断层摄影）能够在不使用造影剂的情况下对软组织内的微结构进行成像。在这里，我们确定了使用这种技术的可行性，在非破坏性检查福尔马林固定的肾组织，从某些仓鼠，自发发展系膜增厚局灶性和节段性肾小球硬化症，并从年龄匹配的叙利亚仓鼠。我们使用了一个三重劳厄情况下的X射线干涉仪与40 μ m厚的分析仪，样品池，和一个X射线电荷耦合器件相机与4.34 μ m的像素大小。肾小球和管状结构的图像与在光学显微镜上使用40-100 x放大倍数观察到的图像相似。在两只雌性肾小球硬化仓鼠的样本中，检测到7个分散的病变。楔形病变表现为肾小管管壁轻度萎缩，管腔明显扩张，肾小球密度增高，Bowman间隙增宽。在没有任何造影剂的标本中，微血管系统清晰可见。因此，相衬显微断层扫描可以检测到小的分散病变的肾脏组织，是一个强大的辅助工具，组织学前的评价。
X-ray crystal interferometer-based X-ray phase-contrast microtomography (phase-contrast microtomography) is able to image microstructures within soft tissue without the use of a contrast agent. Here we determined the feasibility of using this technique in the non-destructive inspection of formalin-fixed kidney tissue from certain hamsters that spontaneously develop mesangial thickening with focal and segmental glomerulosclerosis, and from age-matched Syrian hamsters. We used a triple Laue-case X-ray interferometer with a 40 mu m-thick analyzer, a sample cell, and an X-ray charge-coupled-device camera with a 4.34 mu m pixel size. Images of glomeruli and tubular structures were similar to those seen using 40-100 x magnification on an optical microscope. In samples from two female glomerulosclerotic hamsters, seven scattered lesions were detected. The wedge-shaped pathological lesions included mild atrophic tubular walls, markedly dilated tubular lumen, high-density glomeruli, and widening of Bowman's space. The microvasculature was distinctly visualized in the specimens without any contrast agents. Hence, phase-contrast microtomography can detect small scattered lesions in diseased kidney tissue and is a powerful auxiliary tool for pre-histological evaluations.