Pathotyping of Escherichia coli isolated from community toilet wastewater and stored drinking water in a slum in Bangladesh
Pathotyping of Escherichia coli isolated from community toilet wastewater and stored drinking water in a slum in Bangladesh
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DOI：
10.1111/lam.12878
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发表时间：
2018-06-01
影响因子：
2.4
通讯作者：
Matsuda, T.
中科院分区：
文献类型：
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作者：
Harada, H.;Fujimori, Y.;Matsuda, T.
本研究调查了孟加拉国城市贫民窟卫生废水和饮用水中大肠杆菌病原菌的发生情况以及这些来源之间的潜在联系。通过多重PCR和双指数测序，对621株从卫生废水和储存饮用水中分离的大肠杆菌进行了检测，根据14个毒力基因将其分为8种病原，并对人类特异性大肠杆菌遗传生物标志物H8进行了评估。污水（18.6%）和饮用水（1.7%）中致病性大肠杆菌的比例差异有统计学意义（P < 0.001）。废水中以stib阳性肠毒素大肠杆菌（ETEC）为主，表明现场人群携带ETEC。饮用水中未检出ETEC，且h8阳性分离菌比例（7.8%）显著低于废水（16.3%）（P = 0.001）。我们的研究结果表明，贫民窟的卫生废水被致病性大肠杆菌严重污染，构成了很大的健康风险。此外，大肠杆菌污染的饮用水不仅可能来自人类，也可能来自其他来源。
This study investigated the occurrence of Escherichia coli pathotypes in sanitary wastewater and drinking water in a Bangladeshi urban slum and the potential associations between these sources. We examined 621 E.coli isolates from sanitary wastewater and stored drinking water by multiplex PCR and dual-index sequencing, classifying them into eight pathotypes based on 14 virulence genes and additionally evaluating the possession of the human-specific E.coli genetic biomarker H8. The proportions of pathogenic E.coli were significantly different (P < 0.001) between wastewater (18.6%) and drinking water (1.7%). StIb-positive enterotoxigenic E.coli (ETEC) were predominant in wastewater, indicating that people in the site carried ETEC. In contrast, no ETEC was present in drinking water and the proportion of H8-positive isolates was significantly smaller (7.8%) than that in wastewater (16.3%) (P = 0.001). Our findings indicate that sanitary wastewater from the slum was heavily contaminated with pathogenic E.coli, posing a great health risk. Furthermore, E.coli contamination of drinking water could be derived from not only human but also other sources.