Changes in biomechanical strain and morphology of rat calvarial sutures and bone after Tgf-β3 inhibition of posterior interfrontal suture fusion.
Changes in biomechanical strain and morphology of rat calvarial sutures and bone after Tgf-β3 inhibition of posterior interfrontal suture fusion.
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Tgf-β3 抑制后额间缝融合后大鼠颅骨缝和骨生物力学应变和形态的变化。
DOI：
10.1002/ar.22474
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Opperman,LynneA
中科院分区：
文献类型：
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作者：
Shibazaki-Yorozuya,Reiko;Wang,Qian;Dechow,PaulC;Maki,Koutaro;Opperman,LynneA
颅面缝是骨生长的前沿，响应和适应生物力学环境。在通畅和融合条件下，缝线在调节颅骨生物力学环境方面的作用知之甚少，尤其是延迟或过早的缝线融合将如何影响颅骨生物力学。已证明TGF-β3可在短期内预防或延迟大鼠颅骨中的缝线融合，但尚不清楚经处理缝线的长期通畅性或其后果。因此，假设TGF-β3具有长期影响，可防止缝线融合，从而改变颅骨生物力学。在这项研究中，将含有3 ng TGF-β3的胶原凝胶手术放置在出生后第9天（P9）大鼠的后额间缝（IFS）浅表和骨膜深处。在P9、P24和P70，通过双侧刺激咀嚼肌测量左顶骨、后IFS和矢状缝的咬合力和应变，之后处死大鼠并对缝线通畅性进行组织学分析。结果表明，TGF-β3处理缝线随时间推移的融合程度低于对照组，并且TGF-β3处理组颅骨的应变模式与对照组不同。尽管咬合力随年龄增加而增加，但咬合力的变化不归因于TGF-β3治疗。这些发现表明，持续存在的专利缝线可能会影响应变模式，也许当较高的咬合力存在于成年动物。Anat Rec，，2012.© 2012 Wiley Periodicals，Inc.
Craniofacial sutures are bone growth fronts that respond and adapt to biomechanical environments. Little is known of the role sutures play in regulating the skull biomechanical environment during patency and fusion conditions, especially how delayed or premature suture fusion will impact skull biomechanics. Tgf‐β3 has been shown to prevent or delay suture fusion over the short term in rat skulls, yet the long‐term patency or its consequences in treated sutures is not known. It was therefore hypothesized that Tgf‐β3 had a long‐term impact to prevent suture fusion and thus alter the skull biomechanics. In this study, collagen gels containing 3 ng Tgf‐β3 were surgically placed superficial to the posterior interfrontal suture (IFS) and deep to the periosteum in postnatal day 9 (P9) rats. At P9, P24, and P70, biting forces and strains over left parietal bone, posterior IFS, and sagittal suture were measured with masticatory muscles bilaterally stimulated, after which the rats were sacrificed and suture patency analyzed histologically. Results demonstrated that Tgf‐β3 treated sutures showed less fusion over time than control groups, and strain patterns in the skulls of the Tgf‐β3‐treated group were different from that of the control group. Although bite force increased with age, no alterations in bite force were attributable to Tgf‐β3 treatment. These findings suggest that the continued presence of patent sutures can affect strain patterns, perhaps when higher bite forces are present as in adult animals. Anat Rec,, 2012. © 2012 Wiley Periodicals, Inc.