3,3′-Diindolylmethane induces anti-human gastric cancer cells by the miR-30e-ATG5 modulating autophagy
3,3′-Diindolylmethane induces anti-human gastric cancer cells by the miR-30e-ATG5 modulating autophagy
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DOI：
10.1016/j.bcp.2016.06.018
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发表时间：
2016-09-01
影响因子：
5.8
通讯作者：
Lu, Rongzhu
中科院分区：
文献类型：
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作者：
Ye, Yang;Fang, Yanfei;Lu, Rongzhu
3,3′-二吲哚甲烷（DIM）是一类从十字花科蔬菜中提取的相对无毒的吲哚衍生物，是一种很有前景的抗癌植物化学物质，但其分子机制尚未完全阐明。在本研究中，我们报道了DIM对人胃癌细胞自噬的一种新的调节。我们发现DIM在体外和体内均有剂量依赖性地抑制胃癌细胞的生长。胃癌细胞中ATG5和LC3被DIM激活。此外，DIM下调miR-30e， miR-30e靶向ATG5的3'-UTR抑制其翻译。综上所述，这些结果表明DIM可能通过miR-30e-ATG5调节自噬抑制胃癌细胞的增殖。(C) 2016 Elsevier Inc.版权所有。
3,3'-Diindolylmethane (DIM), a class of relatively non-toxic indole derivatives from cruciferous vegetables, has been reported as a promising anticancer phytochemical, but the underlying molecular mechanism is not completely elucidated. In the present study we report a novel regulation of autophagy by DIM in human gastric cancer cells. We found that DIM dose-dependently inhibited the growth of gastric cancer cells in vitro and in vivo. Moreover, ATG5 and LC3 were activated by DIM in gastric cancer cells. Furthermore, miR-30e was down-regulated by DIM and miR-30e targeted the 3'-UTR of ATG5 to inhibit its translation. Overall, these results suggest that DIM may through the miR-30e-ATG5 modulating autophagy inhibit the proliferation of gastric cancer cells. (C) 2016 Elsevier Inc. All rights reserved.