Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology
Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology
复制标题
DOI：
10.1128/jcm.01475-16
复制
发表时间：
2016-11-01
影响因子：
9.4
通讯作者：
Bankowski, Matthew J.
中科院分区：
文献类型：
--
作者：
Hayden, Randall T.;Clinton, Lani K.;Bankowski, Matthew J.
抗生素敏感性试验的延迟报告仍然是细菌感染治疗的临床决策的限制因素。本研究评估了使用前向激光散射（FLLS）来测量细菌生长，以早期确定抗菌药物敏感性。使用两种市售抗菌检测系统（Vitek 2和MicroScan MIC panel）对金黄色葡萄球菌、大肠埃希菌和铜绿假单胞菌的三种分离株（两种临床分离株和一种参考菌株）进行一式三份检测，以挑战BacterioScan FLLS。BacterioScan FLLS与商业方法显示出高度的分类一致性。与用作参比标准品的每个商业系统进行的成对比较显示，与MicroScan的一致率为88.9%（两个微小错误），与Vitek的一致率为72.2%（五个微小错误）。使用BacterioScan系统的FLLS显示出作为快速准确测定抗菌药物敏感性的新方法的前景。
The delayed reporting of antimicrobial susceptibility testing remains a limiting factor in clinical decision-making in the treatment of bacterial infection. This study evaluates the use of forward laser light scatter (FLLS) to measure bacterial growth for the early determination of antimicrobial susceptibility. Three isolates each (two clinical isolates and one reference strain) of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were tested in triplicate using two commercial antimicrobial testing systems, the Vitek2 and the MicroScan MIC panel, to challenge the BacterioScan FLLS. The BacterioScan FLLS showed a high degree of categorical concordance with the commercial methods. Pairwise comparison with each commercial system serving as a reference standard showed 88.9% agreement with MicroScan (two minor errors) and 72.2% agreement with Vitek (five minor errors). FLLS using the BacterioScan system shows promise as a novel method for the rapid and accurate determination of antimicrobial susceptibility.