Low-intensity pulsed ultrasound promotes the formation of periodontal ligament stem cell sheets and ectopic periodontal tissue regeneration
Low-intensity pulsed ultrasound promotes the formation of periodontal ligament stem cell sheets and ectopic periodontal tissue regeneration
复制标题
低强度脉冲超声促进牙周膜干细胞片的形成和异位牙周组织再生。
DOI：
10.1002/jbm.a.37102
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发表时间：
2020-09-30
影响因子：
4.9
通讯作者：
Song, Jinlin
中科院分区：
文献类型：
--
作者：
Li, Han;Zhou, Jie;Song, Jinlin
人牙周膜干细胞（human periodontal ligament stem cells，hPDLSCs）在牙周组织工程中发挥着重要作用。低强度脉冲超声波（LIPUS）是一种有效的刺激物，可以调节细胞的生物学行为。本研究旨在探索LIPUS促进hPDLSC片层（hPDLSCS）形成和功能的潜力。采用苏木精-伊红（H&E）染色、western blot、real-time PCR、碱性磷酸酶（ALP）、茜素红染色等方法观察LIPUS对hPDLSCs的体外成骨作用。将含或不含hPDLSCS的羟基磷灰石移植到裸鼠背部的皮下口袋中，并进行组织学分析。H&E染色显示细胞外基质（ECM）的合成增加，实时PCR检测到LIPUS处理后ECM相关基因的显著增加。此外，LIPUS可促进成骨分化相关基因和蛋白的表达。ALP和茜素红染色也发现LIPUS促进hPDLSCs的成骨。体内移植后，再生出更致密的类似于牙周膜的胶原纤维。总之，这些结果表明，LIPUS不仅促进hPDLSCs的形成和成骨分化，而且是牙周组织工程的潜在治疗策略。
Human periodontal ligament stem cells (hPDLSCs) sheets play an important role in periodontal tissue engineering. Low-intensity pulsed ultrasound (LIPUS) has been reported as an effective stimulus to regulate cell biological behavior. The present study aims to explore the potential of LIPUS to promote the formation and function of hPDLSC sheets (hPDLSCSs). Hematoxylin-eosin (H&E) staining, western blot, real-time PCR, alkaline phosphatase (ALP), and alizarin red staining were used to evaluate the formation and osteogenic effect of LIPUS on hPDLSCSsin vitro. Hydroxyapatite with or without hPDLSCSs was transplanted in the subcutaneous pockets on the back of nude mice and histological analysis was performed. H&E staining showed increased synthesis of extracellular matrix (ECM) and real-time PCR detected a significant increase in ECM-related genes after LIPUS treatment. In addition, LIPUS could promote the expression of osteogenic differentiation-related genes and proteins. ALP and alizarin red staining also found LIPUS enhanced the osteogenesis of hPDLSCSs. After transplantationin vivo, more dense collagen fibers similar to periodontal ligament were regenerated. Collectively, these results indicate that LIPUS not only promotes the formation and osteogenic differentiation of hPDLSCSs but also is a potential treatment strategy for periodontal tissue engineering.