Naringenin suppresses Edwardsiella tarda infection in GAKS cells by NanA sialidase inhibition
Naringenin suppresses Edwardsiella tarda infection in GAKS cells by NanA sialidase inhibition
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柚皮素通过 NanA 唾液酸酶抑制 GAKS 细胞中的迟缓爱德华氏菌感染
DOI：
10.1016/j.fsi.2016.12.018
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
K. Shiozaki
中科院分区：
文献类型：
--
作者：
Shinyoshi S.;Y. Kamada;K. Matsusaki;P.K. Chigwechokha;S. Tepparin;K. Araki;M. Komatsu;K. Shiozaki
迟缓爱德华氏菌(Edwardsiella Tarda)是引起水产养殖业爱德华氏菌病的革兰氏阴性细菌。以前的研究表明，迟发性纳米A唾液酸酶通过抑制鱼类细胞中的糖蛋白而在感染过程中发挥关键作用。另一方面，经典的唾液酸酶抑制剂2-脱氧-2，3-脱氢-N-乙酰神经氨酸对金鱼鳞片GAKS细胞的感染具有负性调节作用。鉴于柑橘提取物已被广泛应用于鱼类饲料中以改善肉类品质，为了建立水产养殖中迟发性埃希氏菌的抑制模型，对柑橘植物化学物质中抑制NaNA活性的可能性进行了评估。一些黄烷酮类化合物如柚皮苷、橙皮苷、橙皮苷和柚皮苷在体外对重组NaNAA具有唾液酸酶抑制活性。其中，柚皮苷元的抑制活性最强，其抑制模式为非竞争性。在200和400kDμM时，柚皮苷元显著抑制GAKs细胞的迟发性感染，而无杀菌作用。另一方面，柚皮苷元的糖基化形式--柚皮苷对GAKS细胞的迟发性感染表现出轻微的抑制作用，提示黄烷酮上的糖苷可能在抑制NANA中起重要作用。荧光显微镜分析证实，柚皮苷元处理能减少迟发性艾美耳球虫GAKS细胞的侵袭数量。本研究证明了在鱼类饲料中添加柚皮苷元作为抑制迟发性感染的有效成分的可能性。
Edwardsiella tarda (E. tarda) is a gram-negative bacterium, which causes Edwardsiellosis in aquaculture. Previous studies indicate thatE. tardaNanA sialidase plays crucial roles in infection through the desialylation of glycoproteins in fish cells. On the other hand, 2-deoxy-2,3-dehydro-N-acetylneuraminic acid, classic sialidase inhibitor, negatively regulatesE. tardainfection of goldfish scale GAKS cells. Here, to development the suppression model ofE. tardainfection for aquaculture application, the possibility of NanA inhibitory activities in citrus phytochemicals was evaluated as citrus extracts have widely been used as a supplement in fish diets for the improvement of meat quality. Some flavanones such as naringenin, hesperetin, hesperidin and naringin showed sialidase inhibitory activity toward recombinant NanAin vitro. Among them, naringenin showed the most potent inhibitory activity and its inhibitory pattern was non-competitive. Naringenin significantly suppressedE. tardainfection in GAKS cells at 200 and 400 μM without bactericidal effect onE. tarda. On the other hand, naringin, glycosylation form of naringenin, showed slight suppression ofE. tardainfection toward GAKS cells, suggesting the glycosides on flavanone could be important for NanA inhibition. Fluorescence microscopy analysis verified that number of invadingE. tardain GAKS cells was declined by naringenin treatment. The present study exhibited the possibility of naringenin as an effective ingredient in fish diet for the inhibition ofE. tardainfection.