Germline transformation of Caenorhabditis elegans by injection.
Germline transformation of Caenorhabditis elegans by injection.
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DOI：
10.1007/978-1-59745-202-1_10
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
Singson, Andrew
中科院分区：
文献类型：
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作者：
Kadandale, Pavan;Chatterjee, Indrani;Singson, Andrew
显微注射是秀丽隐杆线虫DNA转化的常用技术。它是将遗传和分子分析与表型分析联系起来的强大工具。在这一章中，我们将提供一个概述显微注射生殖系转化蠕虫。我们的讨论将着重于C.线虫生殖生物学、应用和进行显微注射以成功获得转基因蠕虫的方案。
Microinjection is a commonly used technique for DNA transformation in Caenorhabditis elegans. It is a powerful tool that links genetic and molecular analysis to phenotypic analysis. In this chapter we shall provide an overview of microinjection for germline transformation in worms. Our discussion will emphasize C. elegans reproductive biology, applications and protocols for carrying out microinjection in order to successfully obtain transgenic worms.