Repeated but not single isoflurane exposure improved the spatial memory of young adult mice
Repeated but not single isoflurane exposure improved the spatial memory of young adult mice
复制标题
重复而非单次异氟烷暴露可改善年轻成年小鼠的空间记忆
DOI：
10.1111/j.1399-6576.2010.02385.x
复制
发表时间：
2011-04-01
影响因子：
2.1
通讯作者：
Wang, X.
中科院分区：
文献类型：
--
作者：
Su, D.;Zhao, Y.;Wang, X.
背景全身麻醉对老年啮齿动物空间记忆的损害，但其在青年啮齿动物空间记忆中的作用尚未得到很好的研究。本研究旨在确定单次和多次异氟醚暴露对年轻成年小鼠空间记忆的影响。方法实验设计分为三个系列。在第一个系列中，2个月大的动物随机暴露于1.4%异氟烷2或6小时，或汽车气体6小时（每组n=15）。麻醉后48 h Morris水迷宫（MWM）测试评估空间记忆。第二部分，连续5天，随机将动物暴露于1.4%异氟醚（异氟醚组，n=15）或仅暴露于汽车气体（对照组，n=15）中，每天2小时。48小时后，进行MWM。第三部分与第二部分一样，将动物随机分为异氟醚组（n=15）和对照组（n=15）。两周后行MWM。结果与对照组相比，异氟醚暴露后48 h的平均逃避潜伏期和平均途径长度显著缩短。然而，在重复异氟烷暴露2周后进行测试时，两组之间没有差异。与对照组相比，单次暴露于异氟醚2或6小时对小鼠在MWM中的表现没有影响。结论反复而非单次异氟醚暴露可暂时改善幼鼠空间记忆。
BackgroundExposure to general anesthesia damages the spatial memory of aged rodents, but its role in the young adult has not been well investigated. This study was conducted to determine the effects of single and repeated isoflurane exposure on spatial memory in young adult mice.MethodsThe experimental design comprised three series of experiments. In the first series, animals (2 months old) were randomly exposed to 1.4% isoflurane for 2 or 6 h or vehicle gas for 6 h (n=15 in each group). The Morris water maze (MWM) test was performed at 48 h after anesthetic exposure to evaluate spatial memory. In the second part, animals were randomly exposed to 1.4% isoflurane (isoflurane group, n=15) or vehicle gas only (control group, n=15) for 2 h/day on 5 consecutive days. Forty-eight hours later, the MWM was performed. In the third part, animals were randomized into an isoflurane group (n=15) and a control group (n=15) as in the second part of the experiment. Two weeks later, the MWM was performed.ResultsThe mean escape latency time and the mean pathway length measured at 48 h after repeated isoflurane exposure decreased significantly compared with the control. However, there was no difference between the two groups when tested at 2 weeks after repeated isoflurane exposure. A single exposure to isoflurane lasting 2 or 6 h had no effect on the performance of mice in the MWM as compared with the control.ConclusionRepeated but not single isoflurane exposure temporarily improved the spatial memory of young adult mice.