Fiber Connections of the Caudal Corpus Cerebelli, with Special Reference to the Intrinsic Circuitry, in a Teleost (Oreochromis niloticus)
Fiber Connections of the Caudal Corpus Cerebelli, with Special Reference to the Intrinsic Circuitry, in a Teleost (Oreochromis niloticus)
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硬骨鱼（Oreochromis niloticus）尾部小脑体的纤维连接，特别是内在电路
DOI：
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Hironobu Ito
中科院分区：
文献类型：
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作者：
K. Imura;N. Yamamoto;M. Yoshimoto;Masato Endo;K. Funakoshi;Hironobu Ito
尼罗罗非鱼小脑体尾部可分为背侧区和腹侧区。已知尾侧小脑体的背侧（dGL）和腹侧（vGL）区域的颗粒细胞层接收由旁连合核中继的来自端脑的间接输入。下行通路的地形组织，和dGL和vGL接收来自不同背端脑部分的输入。而小脑尾侧体则投射小脑外传出神经。然而，它仍然是未知的下行端脑输入是如何在小脑内处理。因此，本研究通过向背侧和腹侧区域的分子层注射神经示踪剂来研究小脑尾侧体的内在联系。将示踪剂注射到腹侧分子层中导致腹侧体的vGL和神经节层中的标记细胞。通过单轴突重建详细分析了神经节层标记细胞的轴突轨迹，这表明末端部分仅限于背体的神经节层。小脑尾侧体外未见标记终末。示踪剂应用到背侧分子层导致标记细胞不仅在背侧体的神经节层和颗粒细胞层，而且在腹侧体的神经节层。后一项发现证实了存在的内在的预测，从腹侧区域的背侧区域的尾侧小脑体。我们进一步揭示了内在投射神经元是浦肯野细胞的免疫组织化学zerosis II（醛缩酶C），这是浦肯野细胞的标志物，结合示踪剂注射到背侧体。与注射到腹侧体，注射到背侧体导致标记的终端在小脑外结构，如内侧纵束和网状结构的核。本研究表明，来自端脑不同部位的间接输入由小脑尾侧体的不同区域接收和处理，并通过背侧体的传出神经元发出体外。
The caudal part of the corpus cerebelli of Nile tilapia can be divided into dorsal and ventral regions. The granule cell layer of the dorsal (dGL) and ventral (vGL) regions of the caudal corpus cerebelli is known to receive indirect inputs from the telencephalon relayed by the nucleus paracommissuralis. The descending pathways are topographically organized, and the dGL and vGL receive inputs from different dorsal telencephalic parts. The caudal corpus cerebelli, in turn, projects extracerebellar efferents. However, it remains unknown how the descending telencephalic inputs are processed within the cerebellum. Therefore, the present study investigated intrinsic connections of the caudal corpus cerebelli by injecting neural tracers into the molecular layer of dorsal and ventral regions. Injections of tracers into the ventral molecular layer resulted in labeled cells in the vGL and the ganglionic layer of the ventral corpus. The axonal trajectories from labeled cells in the ganglionic layer were analyzed in detail via single-axon reconstructions, which suggested that the terminal portions were confined to the ganglionic layer of the dorsal corpus. No labeled terminals were observed outside the caudal corpus cerebelli. Tracer applications to the dorsal molecular layer resulted in labeled cells not only in the ganglionic layer and the granule cell layer of the dorsal corpus but also in the ganglionic layer of the ventral corpus. The latter finding confirms the presence of intrinsic projections from the ventral region to the dorsal region in the caudal corpus cerebelli. We further revealed that the intrinsic projection neurons are Purkinje cells by immunohistochemistry for zebrin II (aldolase C), which is a marker of Purkinje cells, combined with tracer injections into the dorsal corpus. Unlike injections into the ventral corpus, injections into the dorsal corpus resulted in labeled terminals in extracerebellar structures, such as the nucleus of the medial longitudinal fascicle and reticular formation. The present study suggests that indirect inputs from different telencephalic parts received and processed by distinct regions of caudal corpus cerebelli are sent out of the corpus through the efferent neurons in the dorsal corpus.
zebrin II 的克隆揭示了其与醛缩酶 C 的同一性。
DOI：
10.1242/dev.120.8.2081
发表时间：
1994
期刊：
Development (Cambridge, England)
影响因子：
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作者：
Ahn,AH;Dziennis,S;Hawkes,R;Herrup,K
通讯作者：
Herrup,K