MOLECULAR EVOLUTION OF INVERSIONS IN DROSOPHILA-PSEUDOOBSCURA - THE AMYLASE GENE REGION
MOLECULAR EVOLUTION OF INVERSIONS IN DROSOPHILA-PSEUDOOBSCURA - THE AMYLASE GENE REGION
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DOI：
10.1073/pnas.88.1.305
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发表时间：
1991-01-01
影响因子：
11.1
通讯作者：
ANDERSON, WW
中科院分区：
文献类型：
--
作者：
AQUADRO, CF;WEAVER, AL;ANDERSON, WW
果蝇第三条染色体的淀粉酶区域已被克隆并定位于细胞学条带 73A。它包含在一系列高度多态性倒置中，并作为倒置基因排列的分子进化分析的便捷工具。假暗色藻中的淀粉酶是一个由三个基因组成的家族，有些染色体上有其中一两个基因的缺失。分离出两个覆盖 26 KB 的重叠克隆，并用作探针来调查 28 个品系中的 DNA 限制性图谱多态性，代表自然群体中发现的五种主要倒位类型，以及来自密切相关物种 Drosophila persimilis 和 Drosophila miranda 的单染色体。不同基因排列之间的限制性位点差异比具有相同基因排列的染色体之间的限制性位点差异大得多。对限制性图谱单倍型进行聚类产生了与独立于细胞遗传学考虑而生成的系统发育相一致的树状图。倒位多态性估计已有约200万年的历史。
The amylase region of the third chromosome of Drosophila pseudoobscura has been cloned and localized to cytological band 73A. It is contained within a series of highly polymorphic inversions and serves as a convenient tool for a molecular evolutionary analysis of the inverted gene arrangements. Amylase in D. pseudoobscura is a family of three genes, and some chromosomes have deletions for one or two of them. Two overlapping clones covering 26 kilobases were isolated and used as probes to survey DNA restriction map polymorphism among 28 lines, representing five of the major inversion types found in natural populations, as well as single chromosomes from the closely related species Drosophila persimilis and Drosophila miranda. Restriction-site differences are considerably greater among the various gene arrangements than among chromosomes with the same gene arrangement. Clustering the restriction map haplotypes yielded a drendrogram concordant with the phylogeny generated independently from cytogenetic considerations. The inversion polymorphism is estimated to be about 2 million years old.