Role of fragmentation activity in cellulose hydrolysis
Role of fragmentation activity in cellulose hydrolysis
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DOI：
10.1016/j.ibiod.2006.06.007
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发表时间：
2006-10
影响因子：
4.8
通讯作者：
Abdul A N Saqib;P. Whitney
中科院分区：
文献类型：
--
作者：
Abdul A N Saqib;P. Whitney
纤维素水解的研究主要集中在纤维素酶系的三个组分上，即内切葡聚糖酶、外切葡聚糖酶和纤维素酶。很少有人注意到某些纤维素分解系统的裂解活性。我们已经注意到，尽管是改性纤维素（CMC）的更强大的降解剂，但疣孢漆斑菌的7天生长培养物滤液在降解纯的未改性纤维素方面不如里氏木霉有效。扫描电子显微镜成像显示，后者的一个显着特点是它的能力，片段（浸渍）的纤维素。纤维素颗粒大小随培养时间的延长而减小。reesei在37 ° C。这被用作预处理。然后将预处理的纤维素洗涤并与新鲜的T. reesei或M.疣孢培养预处理增加了T. reesei培养物滤液中，但随后在M.疣孢培养滤液。据推测，预处理的片段化活性打开了进一步水解的攻击位点，但这些位点不可用于其他酶系统的攻击。
Most studies of cellulose hydrolysis have been carried out on three components of the cellulolytic systems, viz, endoglucanases, exoglucanases, and cellobiases. Little attention has been paid to the fragmentation activity of certain cellulolytic systems. We have noticed that despite being a more powerful degrader of modified cellulose (CMC), the 7-day grown culture filtrate of Myrothecium verrucaria was less effective than that of Trichoderma reesei at degrading pure unmodified cellulose. Scanning electron microscopy imaging showed that one distinguishing feature of the latter is its ability to fragment (macerate) the cellulose. Cellulose particle size decreased with time as it was incubated in the culture filtrate of T. reesei at 37°C. This was used as a pre-treatment. Pre-treated cellulose was then washed and incubated with fresh T. reesei or M. verrucaria culture filtrates. Pre-treatment increased liberation of reducing sugars during subsequent incubation of cellulose in T. reesei culture filtrate but not in subsequent incubation in M. verrucaria culture filtrate. It was hypothesized that fragmentation activity of the pre-treatment opened up attack sites for further hydrolysis, but these were not available for attack by other enzyme systems.