Functional analysis of Aarf domain-containing kinase 1 in Drosophila melanogaster.
Functional analysis of Aarf domain-containing kinase 1 in Drosophila melanogaster.
复制标题
果蝇中含有 Aarf 结构域的激酶 1 的功能分析。
DOI：
10.1002/dvdy.66
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Thummel,CarlS
中科院分区：
文献类型：
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作者：
Wisidagama,DonaR;Thomas,StefanM;Lam,Geanette;Thummel,CarlS
背景ADCK蛋白是一种预测的线粒体激酶。这些蛋白质的大多数研究都集中在Abc 1/Coq 8亚家族，这有助于辅酶Q的生物合成。与此相反，鲜为人知的是ADCK 1，尽管它在酵母，果蝇，秀丽隐杆线虫和mammalia.ResultsWe表明，果蝇ADCK 1突变体死亡的二龄幼虫的双口钩和气管断裂的进化保守。组织特异性基因拯救和RNAi研究表明，ADCK 1在气管中对于幼虫的生存力是必要和充分的。此外，气管拯救ADCK 1突变的成年人寿命缩短，发育迟缓，有减少的身体大小，和正常水平的基本metabolites.ConclusionThe幼虫致死率和双口钩看到inADCK 1突变体往往与类固醇激素蜕皮激素水平降低，这表明该基因可能有助于控制蜕皮激素水平或生物利用度。同样，这些动物的气管缺陷可能是由于细胞内脂质运输缺陷引起的。这些ADCK 1的研究提供了一个新的背景下，以确定这个鲜为人知的预测线粒体蛋白的ADCK家族成员的生理功能。
BackgroundThe ADCK proteins are predicted mitochondrial kinases. Most studies of these proteins have focused on the Abc1/Coq8 subfamily, which contributes to Coenzyme Q biosynthesis. In contrast, little is known about ADCK1 despite its evolutionary conservation in yeast,Drosophila,Caenorhabditis elegansand mammals.ResultsWe show thatDrosophila ADCK1mutants die as second instar larvae with double mouth hooks and tracheal breaks. Tissue‐specific genetic rescue and RNAi studies show thatADCK1is necessary and sufficient in the trachea for larval viability. In addition, tracheal‐rescuedADCK1mutant adults have reduced lifespan, are developmentally delayed, have reduced body size, and normal levels of basic metabolites.ConclusionThe larval lethality and double mouth hooks seen inADCK1mutants are often associated with reduced levels of the steroid hormone ecdysone, suggesting that this gene could contribute to controlling ecdysone levels or bioavailability. Similarly, the tracheal defects in these animals could arise from defects in intracellular lipid trafficking. These studies ofADCK1provide a new context to define the physiological functions of this poorly understood member of the ADCK family of predicted mitochondrial proteins.