Development of a backward-mode photoacoustic microscope using a Fabry-Pérot sensor
Development of a backward-mode photoacoustic microscope using a Fabry-Pérot sensor
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使用法布里-珀罗传感器开发后向模式光声显微镜
DOI：
10.1117/12.2525785
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Jan Laufer
中科院分区：
文献类型：
--
作者：
Ulrike Pohle;Elisabeth Baumann;Silvio Pulwer;Claus Villringer;Edward Zhang;Holger Gerhardt;Jan Laufer
光学分辨率光声显微镜（PAM）已被证明能够获得高分辨率（μm）的功能和解剖图像。对于后向模式操作，传统的压电超声换能器由于其不透明性和尺寸而需要远离信号源放置。这可能导致降低的声学灵敏度。平面Fabry-Perot聚合物薄膜干涉仪（FPI）传感器具有克服这一限制的潜力，因为它们对激发波长是透明的，可以紧邻信号源放置以获得高声学灵敏度，并且提供宽带频率响应（0 - 50 MHz）。在这项研究中，我们提出了一个高帧率，后向模式OR-PAM系统的基础上，平面FPI超声传感器。ns脉冲激光器提供激发脉冲（<200 nJ，最大脉冲重复频率= 200 kHz，532 nm）以产生光声波，使用在765-781 nm处询问的平面FPI传感器检测光声波。对于后向模式操作和最高的声学灵敏度，激励和询问光束同轴对准和光栅扫描。对传感器的光学传递函数、空间分辨率和检测灵敏度进行了测试。获得了叶片模型的图像和斑马鱼幼虫的第一个体内图像。这种方法将使快速3D OR-PAM具有高分辨率和高灵敏度，用于功能和分子成像应用。基于FPI的超声检测也有可能实现双模式光学和声学分辨率PAM以及光声成像与纯光学模式（如多光子显微镜）的集成。
Optical-resolution photoacoustic microscopy (PAM) has been shown to enable the acquisition of high resolution (μm) functional and anatomical images. For backward-mode operation, conventional piezoelectric ultrasound transducers need to be placed far away from the signal source due to their opacity and size. This can result in reduced acoustic sensitivity. Planar Fabry-Perot polymer film interferometer (FPI) sensors have the potential to overcome this limitation since they are transparent to the excitation wavelength, can be placed immediately adjacent to the signal source for high acoustic sensitivity, and offer a broadband frequency response (0 –50 MHz). In this study, we present a high frame rate, backward-mode OR-PAM system based on a planar FPI ultrasound sensor. A ns-pulsed laser provides excitation pulses (<200 nJ, maximum pulse repetition frequency = 200 kHz, 532 nm) to generate photoacoustic waves that are detected using a planar FPI sensor interrogated at 765-781 nm. For backwardmode operation and highest acoustic sensitivity, the excitation and interrogation beams are coaxially aligned and rasterscanned. The optical transfer function of the sensor, the spatial resolution and the detection sensitivity were determined to characterise the set-up. Images of a leaf phantom and first in vivo images of zebrafish larvae were acquired. This approach will enable fast 3D OR-PAM with high resolution and high sensitivity for functional and molecular imaging applications. FPI-based ultrasound detection also has the potential to enable dual-mode optical- and acousticresolution PAM and the integration of photoacoustic imaging with purely optical modalities such as multi-photon microscopy.
影响因子：
1.9
作者：
Buchmann, Jens;Guggenheim, James;Laufer, Jan
通讯作者：
Laufer, Jan