HSP104 REQUIRED FOR INDUCED THERMOTOLERANCE
HSP104 REQUIRED FOR INDUCED THERMOTOLERANCE
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DOI：
10.1126/science.2188365
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发表时间：
1990-06-01
期刊：
影响因子：
56.9
通讯作者：
LINDQUIST, SL
中科院分区：
文献类型：
--
作者：
SANCHEZ, Y;LINDQUIST, SL
从酵母菌中分离到一个热休克蛋白基因HSP 104，并对其进行了缺失突变。突变细胞以与野生型细胞相同的速度生长，并且在直接暴露于高温时以相同的速度死亡。然而，当给予温和的预热处理时，突变细胞没有获得对热的耐受性，就像野生型细胞一样。用野生型基因转化挽救了突变细胞的缺陷。结果表明，一种特殊的热休克蛋白在极端温度下的细胞存活中起着关键作用。
A heat shock protein gene,HSP104, was isolated fromSaccharomyces cerevisiaeand a deletion mutation was introduced into yeast cells. Mutant cells grew at the same rate as wild-type cells and died at the same rate when exposed directly to high temperatures. However, when given a mild pre-heat treatment, the mutant cells did not acquire tolerance to heat, as did wild-type cells. Transformation with the wild-type gene rescued the defect of mutant cells. The results demonstrate that a particular heat shock protein plays a critical role in cell survival at extreme temperatures.