Elevated Circulating CD4+CD25+CD127-/low Regulatory T Cells in Patients with Non-asthmatic Eosinophilic Bronchitis
Elevated Circulating CD4+CD25+CD127-/low Regulatory T Cells in Patients with Non-asthmatic Eosinophilic Bronchitis
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非哮喘性嗜酸性支气管炎患者循环 CD4 CD25 CD127 升高/调节性 T 细胞降低
DOI：
10.1007/s00408-020-00358-x
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发表时间：
2020-05-04
期刊：
影响因子：
5
通讯作者：
Chen, Ruchong
中科院分区：
文献类型：
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作者：
Huang, Jieru;Liu, Jiaxing;Chen, Ruchong
目的非哮喘性嗜酸粒细胞性支气管炎（NAEB）是慢性咳嗽的常见病因。其特征是痰嗜酸性粒细胞增多，类似哮喘，但缺乏气道高反应性。调节性T细胞（Regulatory T cells，TCFs）被认为是免疫抑制因子，参与哮喘的发病机制。然而，TdR和NAEB之间的关系仍然未知。本研究旨在通过比较哮喘患者和健康对照者的循环Tcl 4水平，初步探讨Tcl 4在NAEB中的作用。方法对所有受试者进行呼出气一氧化氮（FeNO）、肺功能测定、支气管激发试验、痰诱导和血常规检查。采用流式细胞术检测外周血单个核细胞的T细胞亚群（CD 4（+）CD 25（+）CD 127（-/低））。并观察了血清TdR水平与临床指标的关系。结果15例NAEB患者、20例哮喘患者和11例健康对照者入选。NAEB组（49.8 ± 18.9 × 10（3）cells/ml）和哮喘组（53.3 ± 18.7 × 10（3）cells/ml）的循环T淋巴细胞绝对数均高于健康对照组（32.7 ± 11.6 × 10（3）cells/ml）（均P < 0.01）。总的来说，TdR与FeNO呈正相关（r = 0.30，P < 0.05）。结论外周血TcR水平的升高反映TcR不仅在哮喘患者中起重要作用，而且在NAEB患者中也起重要作用。
Purpose Non-asthmatic eosinophilic bronchitis (NAEB) is a common cause of chronic cough. It is characterized by sputum eosinophilia like asthma but lacks airway hyperresponsiveness. Regulatory T cells (Tregs) are recognized as immune suppressors and are involved in the pathogenesis of asthma. However, the relationship between Tregs and NAEB remains unknown. This study aimed to preliminarily explore the role of Tregs in NAEB by comparing circulating Tregs levels to asthma and healthy controls. Methods Fractional exhaled nitric oxide (FeNO), spirometry with bronchial provocation test, sputum induction and blood routine test were performed in all subjects. Peripheral blood mononuclear cells were used to detect the Tregs (CD4(+)CD25(+)CD127(-/low)) by flow cytometry. Relationship between the levels of circulating Tregs and clinical indexes was also observed. Results A total of 15 patients with NAEB, 20 patients with asthma and 11 healthy controls were included. The absolute numbers of circulating Tregs in the NAEB group (49.8 +/- 18.9 x 10(3) cells/ml) and asthma group (53.3 +/- 18.7 x 10(3) cells/ml) were higher than that in healthy control group (32.7 +/- 11.6 x 10(3) cells/ml) (both P < 0.01). In total, the level of circulating Tregs showed positive correlation with FeNO (r = 0.30, P < 0.05). Conclusion Tregs may play a key role not only in asthmatic patients, but also in patients with NAEB, as reflected by the elevated Tregs in peripheral blood.