Transfer profile of intramuscularly administered tetrodotoxin to artificial hybrid specimens of pufferfish, Takifugu rubripes and Takifugu niphobles
Transfer profile of intramuscularly administered tetrodotoxin to artificial hybrid specimens of pufferfish, Takifugu rubripes and Takifugu niphobles
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DOI：
10.1016/j.toxicon.2011.08.019
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发表时间：
2011-11-01
期刊：
影响因子：
2.8
通讯作者：
Arakawa, Osamu
中科院分区：
文献类型：
--
作者：
Wang, Junjie;Araki, Taiichiro;Arakawa, Osamu
以红鳍东方鲀（Takifugu rubripes）和黑鳍东方鲀（Takifugu niphobles）人工杂交鱼为材料，通过肌肉注射河豚毒素（Tetrodotoxin，TTX），研究了毒素在杂交鱼体内的蓄积情况和TTX在河豚鱼体内的转移/蓄积情况。在给予溶于生理盐水的146 MU TTX的试验鱼中，TTX通过血液迅速从肌肉转移到其他器官。毒素转移到卵巢迅速增加到53.5 MU/g组织在72小时的测试期结束。肝脏和皮肤中的TTX含量最多约为4-6 MU/g组织，睾丸中的含量低于0.01 MU/g组织。另一方面，基于每个个体的毒素总量（所施用毒素的%），皮肤和肝脏中含有较高的量（分别为20-54%和2- 24%），但肝脏中的量在8-12小时后迅速下降，并在48小时后降至卵巢中的水平以下。这些发现表明，部分TTX首先在肝脏中被吸收，然后在男性样本的皮肤和女性样本的卵巢中转移/积累。(C)2011爱思唯尔有限公司保留所有权利。
Tetrodotoxin (TTX) was intramuscularly administered to artificially hybridized specimens of the pufferfish Takifugu rubripes and Takifugu niphobles to investigate toxin accumulation in hybrids, and TTX transfer/accumulation profiles in the pufferfish body. In the test fish administered 146 MU TTX in physiologic saline, TTX rapidly transferred from the muscle via the blood to other organs. Toxin transfer to the ovary rapidly increased to 53.5 MU/g tissue at the end of the 72-h test period. The TTX content in the liver and skin was, at most, around 4-6 MU/g tissue, and in the testis it was less than 0.01 MU/g tissue. On the other hand, based on the total amount of toxin per individual (% of the administered toxin), the skin and the liver contained higher amounts (20-54% and 2-24%, respectively), but the amount in the liver rapidly decreased after 8-12 h, and fell below the level in the ovary after 48 h. These findings suggest that part of the TTX is first taken up in the liver and then transferred/accumulated in the skin in male specimens and in the ovary in female specimens. (C) 2011 Elsevier Ltd. All rights reserved.