Detection of Epstein-Barr virus-associated antigens and DNA in salivary gland biopsies from patients with Sjogren's syndrome.
Detection of Epstein-Barr virus-associated antigens and DNA in salivary gland biopsies from patients with Sjogren's syndrome.
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DOI：
10.4049/jimmunol.137.10.3162
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发表时间：
1986-11
影响因子：
4.4
通讯作者：
R. Fox;G. Pearson;J. Vaughan
中科院分区：
文献类型：
--
作者：
R. Fox;G. Pearson;J. Vaughan
干燥综合征（SS）是一种以唾液腺和泪腺淋巴细胞浸润为特征的自身免疫性疾病。为了确定EB病毒（EBV）是否可能在这种疾病的发病机制中发挥作用，我们使用单克隆抗体和DNA探针检测这些患者的组织活检和唾液中病毒基因产物和基因组的证据。上皮细胞的细胞质染色（即，导管和/或腺泡细胞）与抗EBV编码的早期抗原（EA-D）的单克隆抗体在8/14例SS患者的唾液腺活检中观察到。该抗体不与正常唾液腺或唾液腺肿瘤反应，也不与SS患者的其他组织反应。SS活检组织中的反应性抗原具有一定的分子量。52，000的EA-D抗原，类似于在用EBV裂解感染的淋巴母细胞中发现的EA-D抗原。在两名SS患者的腮腺活检中检测到EBV DNA，其量为每20微克细胞DNA 0.1至3 pg。Southern印迹用于证明与BamV、Eco D和BamM探针的反应性。8/20例SS患者的腮腺唾液样本含有可通过狭缝印迹杂交检测到的EBV DNA。在年龄匹配的对照组、无干燥症状的类风湿关节炎患者或良性腮腺肿瘤患者的唾液中未检测到EBV DNA。唾液腺和唾液样本中EBV的存在与临床上更严重的SS相关，表现为腺外症状，如血管炎、“假淋巴瘤”的发生和免疫球蛋白水平的显著异常。这些结果表明SS患者唾液腺中EBV含量升高，并表明该病毒可能在发病机制中起作用。
Sjogren's syndrome (SS) is an autoimmune disorder characterized by lymphocytic infiltration of salivary and lacrimal glands. To determine whether Epstein-Barr virus (EBV) might play a role in the pathogenesis of this disorder, we used monoclonal antibodies and DNA probes to detect evidence of viral gene products and genomes in these patients' tissue biopsies and saliva. Cytoplasmic staining of epithelial cells (i.e., ductal and/or acinar cells) with monoclonal antibody against the EBV-encoded early antigen (EA-D) was noted in 8/14 salivary gland biopsies from SS patients. This antibody did not react with normal salivary glands or salivary gland tumors, nor with other tissues from SS patients. The reactive antigen in SS biopsies had a m.w. of 52,000 on the basis of immunoblotting experiments, similar to the EA-D antigen found in lymphoblastoid cells lytically infected with EBV. EBV DNA was detected in parotid biopsies from two SS patients in amounts ranging from 0.1 to 3 pg per 20 micrograms of cellular DNA. Southern blotting was used to demonstrate the reactivity with Bam V, Eco D, and Bam M probes. Parotid saliva samples from 8/20 SS patients contained EBV DNA detectable by slot blot hybridization. EBV DNA was not detected in saliva of age-matched controls, rheumatoid arthritis patients lacking sicca symptoms, or patients with benign parotid tumors. The presence of EBV in salivary gland and saliva samples was associated with clinically more severe SS, as manifested by extraglandular symptoms such as vasculitis, occurrence of "pseudolymphoma", and marked abnormalities in immunoglobulin levels. These results demonstrate an elevated content of EBV in salivary glands of SS patients, and suggest that this virus may play a role in pathogenesis.