Transcriptome analysis.
Transcriptome analysis.
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DOI：
10.1007/10_2011_102
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
F. Stahl;B. Hitzmann;Kai-Oliver Mutz;D. Landgrebe;M. Lübbecke;C. Kasper;J. Walter;T. Scheper
中科院分区：
文献类型：
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作者：
F. Stahl;B. Hitzmann;Kai-Oliver Mutz;D. Landgrebe;M. Lübbecke;C. Kasper;J. Walter;T. Scheper
转录组分析技术是研究和优化哺乳动物细胞培养的重要系统生物学方法。对于重组蛋白的生产，了解影响基因的表达条件是通过基因组工程改良细胞系的主要问题。本章介绍了转录组分析的两种主要技术：微阵列技术和下一代测序。蛋白质为基础的方法也简要概述。此外，这些技术对哺乳动物细胞培养改进的影响进行了讨论。
Transcriptome analysis technologies are important systems-biology methods for the investigation and optimization of mammalian cell cultures concerning with regard to growth rates and productivity. For the production of recombinant proteins, knowledge of the expression conditions of the influencing genes is a major issue in the improvement of cell lines by means of genome engineering. This chapter presents two main techniques for transcriptome analysis: microarray technology and next-generation sequencing. Protein-based methods are also briefly outlined. Furthermore, the impact of these technologies on mammalian cell culture improvement is discussed.