AN INVITRO STUDY OF NEUTROPHILS OBTAINED FROM THE NORMAL GINGIVAL SULCUS
AN INVITRO STUDY OF NEUTROPHILS OBTAINED FROM THE NORMAL GINGIVAL SULCUS
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DOI：
10.1111/j.1600-0765.1982.tb01183.x
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发表时间：
1982-01-01
影响因子：
3.5
通讯作者：
MERGENHAGEN, SE
中科院分区：
文献类型：
--
作者：
CHARON, JA;METZGER, Z;MERGENHAGEN, SE
Five of the major characteristics of peripheral blood neutrophils (PB-PMN) (adherence, chemotaxis, phagocytosis, oxidative metabolism and lysosomal granules) those of normal crevicular neutrophils (CR-PMN)isolated from the same individuals. The presence of Fc and complement component 3b receptors and the production of superoxide were similar in CR-PMN and PB-PMN. Healthy gingival sulci harbored a high percentage of stimulated PMN as determined by the nitroblue tetrazolium (NBT) reduction assay. The percentage of CR-PMN which phagocytized opsonized red blood cells (RBC) was lower than that of PB-PMN. The in vitro migration of CR-PMN was reduced as compared to that of PB-PMN. Crevicular neutrophlis had a diminished ability to adhere to glass surfaces which may be related to a direct non-cytotoxic effect of gingival fluid component(s) on CR-PMN. Morphologic evidence indicated that specific granules are more depleted than azurophil granules in CR-PMN. Interpretation of studies of neutrophil functions in periodontal diseases must consider the observed differences between normal peripheral blood and crevicular neutrophils.
