Rnd3 induces stress fibres in endothelial cells through RhoB.
Rnd3 induces stress fibres in endothelial cells through RhoB.
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DOI：
10.1242/bio.20123574
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发表时间：
2013-02-15
期刊：
影响因子：
2.4
通讯作者：
Ridley AJ
中科院分区：
文献类型：
--
作者：
Gottesbühren U;Garg R;Riou P;McColl B;Brayson D;Ridley AJ
Rnd蛋白是非典型的Rho家族蛋白，其不水解GTP，而是通过表达水平和翻译后修饰来调节。Rnd 1和Rnd 3/RhoE在多种细胞类型中诱导肌动蛋白应力纤维和细胞变圆的损失，而对Rnd 2的反应则更加多变。在这里，我们报告的反应，内皮细胞的RND蛋白。Rnd 3诱导应力纤维的非常短暂的减少，但随后刺激应力纤维的强烈增加，与在其他细胞类型中观察到的减少相反。Rnd 2还增加应力纤维，而Rnd 1诱导应力纤维的损失和细胞-细胞连接的弱化。rnd 3不通过任何已知的信号伴侣起作用，也不需要与膜结合来增加应力纤维。相反，它通过增加RhoB表达起作用，这是Rnd 3诱导的应力纤维组装所必需的。Rnd 2也增加RhoB水平。这些数据表明，Rnd 3表达的细胞骨架响应依赖于细胞类型和上下文，并确定RhoB的调节作为Rnd蛋白影响肌动蛋白细胞骨架的新机制。
Rnd proteins are atypical Rho family proteins that do not hydrolyse GTP and are instead regulated by expression levels and post-translational modifications. Rnd1 and Rnd3/RhoE induce loss of actin stress fibres and cell rounding in multiple cell types, whereas responses to Rnd2 are more variable. Here we report the responses of endothelial cells to Rnd proteins. Rnd3 induces a very transient decrease in stress fibres but subsequently stimulates a strong increase in stress fibres, in contrast to the reduction observed in other cell types. Rnd2 also increases stress fibres whereas Rnd1 induces a loss of stress fibres and weakening of cell–cell junctions. Rnd3 does not act through any of its known signalling partners and does not need to associate with membranes to increase stress fibres. Instead, it acts by increasing RhoB expression, which is then required for Rnd3-induced stress fibre assembly. Rnd2 also increases RhoB levels. These data indicate that the cytoskeletal response to Rnd3 expression is dependent on cell type and context, and identify regulation of RhoB as a new mechanism for Rnd proteins to affect the actin cytoskeleton.
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DOI：
10.1042/bj20082377
发表时间：
2009-10-23
期刊：
The Biochemical journal
影响因子：
--
作者：
Madigan JP;Bodemann BO;Brady DC;Dewar BJ;Keller PJ;Leitges M;Philips MR;Ridley AJ;Der CJ;Cox AD
通讯作者：
Cox AD
DOI：
10.1161/atvbaha.112.252080
发表时间：
2012-08-01
影响因子：
8.7
作者：
Fernandez-Martin, Laura;Marcos-Ramiro, Beatriz;Millan, Jaime
通讯作者：
Millan, Jaime
影响因子：
5.3
作者：
Riento, K;Guasch, RM;Ridley, AJ
通讯作者：
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影响因子：
64.8
作者：
Heng, Julian Ik-Tsen;Nguyen, Laurent;Guillemot, Francois
通讯作者：
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影响因子：
4.8
作者：
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Zheng, Yi